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Retinal Development

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Chai-An Mao

    1. Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2092)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Investigating Neurogenesis in Birds

    • Tania Rodrigues, Laurent Brodier, Jean-Marc Matter
    Pages 1-18

  3. Studying In Vivo Retinal Progenitor Cell Proliferation in Xenopus laevis

    • Cindy X. Kha, Dylan J. Guerin, Kelly Ai-Sun Tseng
    Pages 19-33

  4. Three-Dimensional Culture of Mouse Eyecups

    • Raven Diacou, Punita Bhansali, Wei Liu
    Pages 35-43

  5. Live Imaging of Mouse Retinal Slices

    • Anthony P. Barrasso, Ross A. Poché
    Pages 45-53

  6. Retinal Strip Culture for Studying Ganglion Cell Axon Growth

    • Masayuki Yamashita
    Pages 55-64

  7. Multiple Approaches for Enhancing Neural Activity to Promote Neurite Outgrowth of Retinal Explants

    • Chuan-Chin Chiao, Chin-I Lin, Meng-Jung Lee
    Pages 65-75

  8. Adeno-Associated Virus as Gene Delivery Vehicle into the Retina

    • Shuyun Deng, Kazuhiro Oka
    Pages 77-90

  9. Transposon-Mediated Stable Suppression of Gene Expression in the Developing Chick Retina

    • Masaru Nakamoto, Chizu Nakamoto
    Pages 91-108

  10. A Simple Guide for Generating BAC Transgenic Animals for Retinal Research

    • Cavit Agca, Christian Grimm
    Pages 109-122

  11. Identification and Characterization of Cis-Regulatory Elements for Photoreceptor-Type-Specific Transcription in ZebraFish

    • Wei Fang, Yi Wen, Xiangyun Wei
    Pages 123-145

  12. Ultrasensitive RNAscope In Situ Hybridization System on Embryonic and Adult Mouse Retinas

    • Takae Kiyama, Chai-An Mao
    Pages 147-158

  13. Single-Cell Capture, RNA-seq, and Transcriptome Analysis from the Neural Retina

    • Rachayata Dharmat, Sangbae Kim, Yumei Li, Rui Chen
    Pages 159-186

  14. Genetically Directed Sparse Labeling System for Anatomical Studies of Retinal Ganglion Cells

    • Leila Jamal, Takae Kiyama, Chai-An Mao
    Pages 187-194

  15. Intracellular Dye Microinjection in Retinal Morphological and Communication Studies

    • Ye Long
    Pages 195-206

  16. Multielectrode Array Recording of Mouse Retinas Transplanted with Stem Cell-Derived Retinal Sheets

    • Hung-Ya Tu, Take Matsuyama
    Pages 207-220

  17. Paired Recording to Study Electrical Coupling Between Photoreceptors in Mouse Retina

    • Nange Jin, Zhijing Zhang, Kimberly A. Mankiewicz, Christophe P. Ribelayga
    Pages 221-230

  18. Back Matter

    Pages 231-235
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Keywords

About this book

This volume details commonly used molecular and cellular techniques and specialized methodologies for studying retina neuronal subtypes and electrophysiology. Chapters describe techniques for anatomical studies of retinal ganglion cell morphology, gap-junction-mediated neuronal connection, multi-electrode array recording on mouse retinas, and paired recording to study the electrical coupling between photoreceptors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Retinal Development: Methods and Protocols aims to provide readers with a set of practical experimental tools to study retinal development, regeneration, and function of mature retinal neurons. Many of the protocols and strategies described in one organism can be easily adapted to applications in different model systems.

Editors and Affiliations

  • Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, USA

    Chai-An Mao

Bibliographic Information

  • Book Title: Retinal Development

  • Book Subtitle: Methods and Protocols

  • Editors: Chai-An Mao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0175-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0174-7Published: 01 December 2019

  • Softcover ISBN: 978-1-0716-0177-8Published: 18 January 2021

  • eBook ISBN: 978-1-0716-0175-4Published: 30 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 235

  • Number of Illustrations: 11 b/w illustrations, 48 illustrations in colour

  • Topics: Developmental Biology, Neurosciences

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