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Xenotransplantation

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2110)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Tools for Molecular Studies in Xenotransplantation

    • Sebastián G. Kuguel, Mireia Uribe-Herranz, Cristina Costa
    Pages 27-45
  3. Specific Detection of Neu5Gc in Animal Tissues by Immunohistochemistry

    • Anu Paul, Shirley Bachar Abramovitch, Vered Padler-Karavani
    Pages 59-72
  4. Cell-Based Assays for Modeling Xenogeneic Immune Responses

    • Kelly Casós, Roberta Sommaggio, Magdiel Pérez-Cruz, Cristina Costa
    Pages 99-113
  5. Determination of Redox Status in Serum

    • Kelly Casós, Cristina Costa, Manuel Galiñanes
    Pages 115-128
  6. Generation of Genetically Modified Mice Using CRISPR/Cas9

    • D. Muñoz-Santos, L. Montoliu, A. Fernández
    Pages 129-138
  7. Xenogeneic Lung Transplantation Models

    • Lars Burdorf, Agnes M. Azimzadeh, Richard N. Pierson III
    Pages 173-196
  8. Liver Xenotransplantation in a Nonhuman Primate Model

    • Nalú Navarro-Alvarez, Parsia A. Vagefi
    Pages 197-211
  9. Intra-bone Bone Marrow Transplantation in Pig-to-Nonhuman Primates for the Induction of Tolerance Across Xenogeneic Barriers

    • Kazuhiko Yamada, Yuichi Ariyoshi, Thomas Pomposelli, Kazuhiro Takeuchi
    Pages 213-225
  10. Studying Xenograft Rejection of Bioprosthetic Heart Valves

    • Rizwan A. Manji, Jacqueline S. Manji
    Pages 227-243
  11. Corneal Xenotransplantation: Anterior Lamellar Keratoplasty

    • Bertrand Vabres, Bernard Vanhove, Gilles Blancho
    Pages 245-251
  12. Rat Model of Intra-articular Chondrocyte Xenotransplantation

    • Maribel Marquina, Magdiel Pérez-Cruz, Cristina Costa
    Pages 253-266
  13. Porcine Hepatocytes: Isolation and Liver Tissue Engineering for Xenotransplantation

    • Yi Li, Qiong Wu, Yujia Wang, Hong Bu, Ji Bao
    Pages 267-287
  14. Pig-to-Macaque Islet Xenotransplantation

    • Suzanne Bertera, Michael F. Knoll, Carmela A. Knoll, David K. C. Cooper, Massimo Trucco, Rita Bottino
    Pages 289-314

About this book

This second edition volume delivers updated and new chapters on xenotransplantation covering a variety of methods. Despite many technological challenges faced by the xenotransplantation field, many major advances have been made in the last three decades. Expert researchers provide characterizations of xenogeneic interactions at the cellular and molecular levels, detailed descriptions of complex transplantation models, as well as attractive new tools for further advancement including 3D cell-culturing systems and genomic editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Thorough and intuitive, Xenotransplantation: Methods and Protocol, Second Edition aids scientists in continuing to study xenotransplantation and its multiple aspects.


Editors and Affiliations

  • Institut d’Investigació Biomèdica de Bellvitge (IDIBELL), Hospital Duran i Reynals, L’Hospitalet de LLobregat, Barcelona, Spain

    Cristina Costa

Bibliographic Information

  • Book Title: Xenotransplantation

  • Book Subtitle: Methods and Protocols

  • Editors: Cristina Costa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0255-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0254-6Published: 31 January 2020

  • Softcover ISBN: 978-1-0716-0257-7Published: 31 January 2021

  • eBook ISBN: 978-1-0716-0255-3Published: 30 January 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 363

  • Number of Illustrations: 16 b/w illustrations, 49 illustrations in colour

  • Topics: Molecular Medicine, Regenerative Medicine/Tissue Engineering

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access