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Stem Cells and Tissue Repair

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Chrissa Kioussi

    1. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, USA

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2155)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Culturing and Manipulating Mouse Embryonic Stem Cells

    • Chrissa Kioussi
    Pages 1-9

  3. Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter

    • Kiel T. Butterfield, Patrick S. McGrath, Chann Makara Han, Igor Kogut, Ganna Bilousova
    Pages 11-21

  4. Engraftable Induced Pluripotent Stem Cell -Derived Neural Precursors for Brain Repair

    • Ourania Zygogianni, Georgia Kouroupi, Era Taoufik, Rebecca Matsas
    Pages 23-39

  5. In Vitro Direct Reprogramming of Mouse and Human Astrocytes to Induced Neurons

    • Katerina Aravantinou-Fatorou, Dimitra Thomaidou
    Pages 41-61

  6. Preparation of Bioscaffolds Delivering Stem Cells for Neural Regeneration

    • Li Yao, Ashley DeBrot
    Pages 63-70

  7. Improved Isolation of Human Vascular Wall –Resident Mesenchymal Stem Cells

    • Diana Klein
    Pages 71-81

  8. In Vitro Generation of Vascular Wall –Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells Through VW-MSC–Specific Gene Transfer

    • Jennifer Steens, Hannes Klump, Diana Klein
    Pages 83-97

  9. Analysis of Tooth Innervation in Microfluidic Coculture Devices

    • Pierfrancesco Pagella, Thimios A. Mitsiadis
    Pages 99-106

  10. Peripheral Nerve Regeneration in a Novel Rat Model of Dysphagia

    • Kiyoshi Sakai, Takeshi Tsuruta, Junna Watanabe, Yukiko Sugimura, Kohei Sakaguchi, Wataru Katagiri et al.
    Pages 107-113

  11. Healing of Full-Thickness Murine Skin Wounds Containing Nanofibers Using Splints for Efficient Reepithelialization and to Avoid Contracture

    • Nilika Bhattacharya, Arup K. Indra, Gitali Ganguli-Indra
    Pages 115-123

  12. Enrichment and Characterization of Human and Murine Pulmonary Mesenchymal Progenitor Cells (MPC )

    • Megan Summers, Karen Helm, Susan M. Majka
    Pages 125-140

  13. Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells

    • Francisco Hernández-Torres, Lara Rodríguez-Outeiriño, Amelia Aránega
    Pages 141-150

  14. Isolation, Culture, Cryopreservation, and Identification of Bovine, Murine, and Human Spermatogonial Stem Cells

    • Pedro M. Aponte
    Pages 151-164

  15. Long-Term Ex Vivo Expansion of Murine Spermatogonial Stem Cells in a Simple Serum-Free Medium

    • Hiroshi Kubota, Kazue Kakiuchi
    Pages 165-182

  16. Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions

    • Sara E. Howden, Melissa H. Little
    Pages 183-192

  17. Painting the Pancreas in Three Dimensions: Whole-Mount Immunofluorescence Method

    • Maricela Maldonado, Jeffrey D. Serrill, Hung-Ping Shih
    Pages 193-200

  18. Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts

    • Hui Zhu, Joy Y. Wu
    Pages 201-212

  19. Back Matter

    Pages 213-214
    Download chapter PDF
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Keywords

About this book

This volume looks at a collection of stem cell and regenerative techniques used by both novice and expert scientists. Chapters cover topics such as tissue repaired by expansion and reprogramming; induced pluripotent stem cells driven in neuronal or vascular differentiation; using mesenchymal stem cells to derive skeletal muscle, osteoblasts, and spermatogonial cells; and the technique of monitoring the development of sub-organ microenvironments in the developing pancreas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Stem Cells and Tissue Repair: Methods and Protocols, Second Edition is a valuable resource that provides readers with the latest descriptions and references for exploring this vast field in regenerative medicine. 


Editors and Affiliations

  • Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, USA

    Chrissa Kioussi

Bibliographic Information

  • Book Title: Stem Cells and Tissue Repair

  • Book Subtitle: Methods and Protocols

  • Editors: Chrissa Kioussi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0655-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0654-4Published: 31 May 2020

  • Softcover ISBN: 978-1-0716-0657-5Published: 31 May 2021

  • eBook ISBN: 978-1-0716-0655-1Published: 31 May 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 214

  • Number of Illustrations: 2 b/w illustrations, 34 illustrations in colour

  • Topics: Stem Cells, Regenerative Medicine/Tissue Engineering

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