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Cardiac Regeneration

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Kenneth D. Poss

    1. Department of Cell Biology, Duke University Medical Center, Durham, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Bernhard Kühn

    1. Division of Cardiology, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2158)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Cardiac Injury Models

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Myocardial Infarction Techniques in Adult Mice

      • Elad Bassat, Dahlia E. Perez, Eldad Tzahor
      Pages 3-21

    3. Apical Resection and Cryoinjury of Neonatal Mouse Heart

      • Hua Shen, Ali Darehzereshki, Henry M. Sucov, Ching-Ling Lien
      Pages 23-32

    4. Left Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice

      • Tilman Ziegler, Karl-Ludwig Laugwitz, Christian Kupatt
      Pages 33-41

    5. Myocardial Infarction in Pigs

      • Andrea Bähr, Nadja Hornaschewitz, Christian Kupatt
      Pages 43-50

    6. Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish

      • Ines J. Marques, Andrés Sanz-Morejón, Nadia Mercader
      Pages 51-62Open Access

    7. Cardiac Resection Injury in Zebrafish

      • Delicia Z. Sheng, Dawei Zheng, Kazu Kikuchi
      Pages 63-69

    8. A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish

      • Fei Sun, Adam R. Shoffner, Kenneth D. Poss
      Pages 71-80

    9. Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration

      • Yijen L. Wu
      Pages 81-106

  3. Ex Vivo and In Vivo Approaches

    1. Front Matter

      Pages 107-107
      Download chapter PDF

    2. Isolation, Culture, and Live-Cell Imaging of Primary Rat Cardiomyocytes

      • Marina Leone, Felix B. Engel
      Pages 109-124

    3. Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes

      • Lu Han, Jocelyn Mich-Basso, Bernhard Kühn
      Pages 125-139

    4. Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells

      • Navid A. Nafissi, Paige DeBenedittis, Michael C. Thomas, Ravi Karra
      Pages 141-153

    5. In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells

      • Jun Xu, Li Wang, Jiandong Liu, Li Qian
      Pages 155-170

    6. Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs

      • Abbigail Helfer, Nenad Bursac
      Pages 171-186

    7. Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells

      • Ling Gao, Jianyi (Jay) Zhang
      Pages 187-197

    8. Isolation and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts

      • Honghai Liu, Kevin Bersell, Bernhard Kühn
      Pages 199-210

    9. Ex Vivo Techniques to Study Heart Regeneration in Zebrafish

      • Sierra Duca, Jingli Cao
      Pages 211-222

    10. Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters

      • Francisco X. Galdos, Adrija K. Darsha, Sharon L. Paige, Sean M. Wu
      Pages 223-240
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Keywords

About this book

This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models andmethods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them.


The chapter “Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Department of Cell Biology, Duke University Medical Center, Durham, USA

    Kenneth D. Poss

  • Division of Cardiology, UPMC Children’s Hospital of Pittsburgh, Pittsburgh, USA

    Bernhard Kühn

Bibliographic Information

  • Book Title: Cardiac Regeneration

  • Book Subtitle: Methods and Protocols

  • Editors: Kenneth D. Poss, Bernhard Kühn

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0668-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0667-4Published: 29 August 2020

  • Softcover ISBN: 978-1-0716-0670-4Published: 04 March 2022

  • eBook ISBN: 978-1-0716-0668-1Published: 28 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 346

  • Number of Illustrations: 14 b/w illustrations, 69 illustrations in colour

  • Topics: Regenerative Medicine/Tissue Engineering, Physiology, Biological Techniques

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