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Volume Microscopy

Multiscale Imaging with Photons, Electrons, and Ions

  • Book
  • © 2020

Overview

Editors:
  1. Irene Wacker

    1. Cryo EM, Centre for Advanced Materials (CAM), Universität Heidelberg, Heidelberg, Germany

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  2. Eric Hummel

    1. Sector Life Science, Carl Zeiss Microscopy GmbH, Oberkochen, Germany, Carl Zeiss, Oberkochen, Germany

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  3. Steffen Burgold

    1. BioQuant, Universität Heidelberg, Heidelberg, Germany, Cryo EM, BioQuant, Universitätsklinikum Heidelberg, Heidelberg, Germany

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  4. Rasmus Schröder

    1. Cryo EM, Centre for Advanced Materials (CAM), Universität Heidelberg, Heidelberg, Germany, Cryo EM, BioQuant, Universitätsklinikum Heidelberg, Heidelberg, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 155)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Correlative Super Resolution and Electron Microscopy to Detect Molecules in Their Native Cellular Context

    • Tyler Ogunmowo, Sumana Raychaudhuri, Grant Kusick, Shuo Li, Shigeki Watanabe
    Pages 1-13

  3. Multicolor Superresolution Microscopy: Revealing the Nano World of Astrocytes In Situ

    • Janosch P. Heller, James P. Reynolds, Dmitri A. Rusakov
    Pages 15-35

  4. High-Resolution Molecular Imaging and Its Applications in Brain and Synapses

    • Nhu T. N. Phan, Silvio O. Rizzoli
    Pages 37-58

  5. Advancing Array Tomography to Study the Fine Ultrastructure of Identified Neurons in Zebrafish (Danio rerio )

    • Marlene Strobel, Frederik Helmprobst, Martin Pauli, Manfred Heckmann, Christina Lillesaar, Christian Stigloher
    Pages 59-78

  6. A Low-Tech Approach to Serial Section Arrays

    • Waldemar Spomer, Andreas Hofmann, Lisa Veith, Ulrich Gengenbach
    Pages 79-96

  7. Large Volumes in Ultrastructural Neuropathology Imaged by Array Tomography of Routine Diagnostic Samples

    • Irene Wacker, Carsten Dittmayer, Marlene Thaler, Rasmus Schröder
    Pages 97-120

  8. Correlative Ultrastructural Analysis of Functionally Modulated Synapses Using Automated Tape-Collecting Ultramicrotome and SEM Array Tomography

    • Ye Sun, Connon Thomas, Takayasu Mikuni, Debbie Guerrero-Given, Ryohei Yasuda, Naomi Kamasawa
    Pages 121-149

  9. Large-Scale Automated Serial Section Imaging with a Multibeam Scanning Electron Microscope

    • Anna Lena Eberle, Tomasz Garbowski
    Pages 151-163

  10. Improving Serial Block Face SEM by Focal Charge Compensation

    • Ann-Katrin Unger, Ralph Neujahr, Chris Hawes, Eric Hummel
    Pages 165-178

  11. Using X-Ray Microscopy to Increase Targeting Accuracy in Serial Block-Face Scanning Electron Microscopy

    • Eric A. Bushong, Sébastien Phan, Mark H. Ellisman
    Pages 179-200

  12. FIBSEM Analysis of Interfaces Between Hard Technical Devices and Soft Neuronal Tissue

    • Antje Biesemeier, Birgit Schröppel, Wilfried Nisch, Claus J. Burkhardt
    Pages 201-220

  13. Transforming FIB-SEM Systems for Large-Volume Connectomics and Cell Biology

    • C. Shan Xu, Song Pang, Kenneth J. Hayworth, Harald F. Hess
    Pages 221-243

  14. Image Processing for Volume Electron Microscopy

    • Jörgen Kornfeld, Fabian Svara, Adrian A. Wanner
    Pages 245-262

  15. Forget About Electron Micrographs: A Novel Guide for Using 3D Models for Quantitative Analysis of Dense Reconstructions

    • Daniya J. Boges, Marco Agus, Pierre Julius Magistretti, Corrado Calì
    Pages 263-304

  16. Back Matter

    Pages 305-306
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Keywords

About this book

This volume discusses different approaches to workflows for large volume electron microscopy - from preparation of samples to their imaging in a variety of microscopes - in some cases also applying correlative techniques. The chapters in this book cover topics such as correlative super resolution and electron microscopy to detect molecules in their native cellular context; low-threshold access to serial section arrays; improving serial blockface SEM by focal charge compensation; FIBSEM analysis of interfaces between hard technical devices and soft neuronal tissue; and image processing for volume electron microscopy. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Volume Microscopy: Multiscale Imaging with Photons, Electrons, and Ions is a valuable resource for novice and expert scientists interested in learning more about this evolving field.

Editors and Affiliations

  • Cryo EM, Centre for Advanced Materials (CAM), Universität Heidelberg, Heidelberg, Germany

    Irene Wacker

  • Sector Life Science, Carl Zeiss Microscopy GmbH, Oberkochen, Germany, Carl Zeiss, Oberkochen, Germany

    Eric Hummel

  • BioQuant, Universität Heidelberg, Heidelberg, Germany, Cryo EM, BioQuant, Universitätsklinikum Heidelberg, Heidelberg, Germany

    Steffen Burgold

  • Cryo EM, Centre for Advanced Materials (CAM), Universität Heidelberg, Heidelberg, Germany, Cryo EM, BioQuant, Universitätsklinikum Heidelberg, Heidelberg, Germany

    Rasmus Schröder

Bibliographic Information

  • Book Title: Volume Microscopy

  • Book Subtitle: Multiscale Imaging with Photons, Electrons, and Ions

  • Editors: Irene Wacker, Eric Hummel, Steffen Burgold, Rasmus Schröder

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-0691-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0690-2Published: 30 June 2020

  • Softcover ISBN: 978-1-0716-0693-3Published: 05 July 2020

  • eBook ISBN: 978-1-0716-0691-9Published: 29 June 2020

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XVII, 306

  • Number of Illustrations: 19 b/w illustrations, 88 illustrations in colour

  • Topics: Neurosciences, Analytical Chemistry, Materials Science, general

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