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Patch Clamp Electrophysiology

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Mark Dallas

    1. University of Reading, Reading, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Damian Bell

    1. Sophion Bioscience A/S, Ballerup, Denmark

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2188)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. An Introduction to Patch Clamp Recording

    • Charlotte L. Hill, Gary J. Stephens
    Pages 1-19

  3. Patch Clamp Technology in the Twenty-First Century

    • Jan Dolzer
    Pages 21-49

  4. Heterologous Expression of Ion Channels in Mammalian Cell Lines

    • Alistair Mathie, Emma L. Veale, Robyn G. Holden
    Pages 51-65

  5. Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers: Next-Generation Instrumentation for Multiple Recordings in Parallel

    • Ekaterina Zaitseva, Alison Obergrussberger, Conrad Weichbrodt, Mordjane Boukhet, Frank Bernhard, Christopher Hein et al.
    Pages 67-92

  6. Perforated Whole-Cell Recordings in Automated Patch Clamp Electrophysiology

    • Kadla R. Rosholm, Kim Boddum, Anders Lindquist
    Pages 93-108

  7. Multielectrode Arrays

    • Russell Burley, Jenna R. M. Harvey
    Pages 109-132

  8. Utilising Automated Electrophysiological Platforms in Epilepsy Research

    • Carol J. Milligan, Svenja Pachernegg
    Pages 133-155

  9. Dynamic Clamp on a Windows PC

    • Lorin S. Milescu, Joël Tabak
    Pages 157-177

  10. Combining Whole-Cell Patch-Clamp Recordings with Single-Cell RNA Sequencing

    • Kashif Mahfooz, Tommas J. Ellender
    Pages 179-189

  11. Bioluminescence Methodology for Ion Channel Studies

    • Paul A. Wadsworth, Aditya K. Singh, Nghi Nguyen, Clifford Stephan, Fernanda Laezza
    Pages 191-228

  12. Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native Neurons

    • Francesco Tamagnini
    Pages 229-242

  13. Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method

    • Timothy W. Church, Matthew G. Gold
    Pages 243-257

  14. In Vivo Patch-Clamp Studies

    • Yi Zhou, He Li, Zhongju Xiao
    Pages 259-271

  15. In Vivo Optogenetics with Stimulus Calibration

    • Luke T. Coddington, Joshua T. Dudman
    Pages 273-283

  16. Single or Double Patch-Clamp Recordings In Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics

    • Jean Simonnet, Louis Richevaux, Desdemona Fricker
    Pages 285-309

  17. Optogenetics and Optical Tools in Automated Patch Clamping

    • Kim Boddum, Peder Skafte-Pedersen, Jean-Francois Rolland, Sandra Wilson
    Pages 311-330

  18. Back Matter

    Pages 331-333
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Keywords

About this book

This volume describes a range of standard and novel methodological approaches used to probe ion channel function across different modalities. Chapters guide readers through methods and protocols from an introduction to the decades old patch clamp method for the ion channel neophyte to more complex, recent protocol advances, such as optogenetics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Patch Clamp Electrophysiology:  Methods and Protocols aims to be a reference guide for current and future ion channel physiologists. 

Editors and Affiliations

  • University of Reading, Reading, UK

    Mark Dallas

  • Sophion Bioscience A/S, Ballerup, Denmark

    Damian Bell

Bibliographic Information

  • Book Title: Patch Clamp Electrophysiology

  • Book Subtitle: Methods and Protocols

  • Editors: Mark Dallas, Damian Bell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0818-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0817-3Published: 30 October 2020

  • Softcover ISBN: 978-1-0716-0820-3Published: 30 October 2021

  • eBook ISBN: 978-1-0716-0818-0Published: 29 October 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 333

  • Number of Illustrations: 17 b/w illustrations, 76 illustrations in colour

  • Topics: Human Physiology, Neurosciences

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