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Arabidopsis Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Jose J. Sanchez-Serrano

    1. Centro Nacional de Biotecnología, CSIC Centro Nacional de Biotecnología, Madrid, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Julio Salinas

    1. Centro de Investigaciones Biologicas, CSIC Madrid Centro de Investigaciones Biologicas, Madrid, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2200)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
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  2. Arabidopsis Resources

    1. Front Matter

      Pages 1-1
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    2. Handling Arabidopsis and Other Brassicaceae: Growth, Preservation of Seeds, Transformation, and Genetic Crosses

      • Christopher Calhoun, Deborah Crist, Emma Knee, Julie Miller, Eva Nagy, David E. Somers
      Pages 3-23

    3. Bioinformatic Tools in Arabidopsis Research

      • G. Alex Mason, Alex Cantó-Pastor, Siobhan M. Brady, Nicholas J. Provart
      Pages 25-89

  3. Genetic Techniques in Arabidopsis

    1. Front Matter

      Pages 91-91
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    2. Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene

      • Carlos Alonso-Blanco, Belén Méndez-Vigo, F. Xavier Picó
      Pages 93-112

    3. Non-sterile Grafting Methods for Arabidopsis

      • Nien-Chen Huang, Tien-Shin Yu
      Pages 113-119

    4. CRISPR/Cas9-Based Genome Editing Toolbox for Arabidopsis thaliana

      • Daisuke Miki, Gaurav Zinta, Wenxin Zhang, Fangnan Peng, Zhengyan Feng, Jian-Kang Zhu
      Pages 121-146

    5. Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration

      • César Bernat-Silvestre, Vanessa De Sousa Vieira, Judit Sánchez-Simarro, Fernando Aniento, María Jesús Marcote
      Pages 147-155

    6. Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts

      • Yonglun Zeng, Changyang Ji, Youshun Lin, Liwen Jiang
      Pages 157-165

    7. Arabidopsis Photosynthetic and Heterotrophic Cell Suspension Cultures

      • Enrico Cortese, Luca Carraretto, Barbara Baldan, Lorella Navazio
      Pages 167-185

    8. Genome-Wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals

      • Tak Lee, Insuk Lee
      Pages 187-210

  4. Molecular and Cell Biological Techniques in Arabidopsis

    1. Front Matter

      Pages 211-211
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    2. Visualizing and Measuring Single Locus Dynamics in Arabidopsis thaliana

      • Anis Meschichi, Stefanie Rosa
      Pages 213-224

    3. Identification and Quantification of Small RNAs

      • Di Sun, Zeyang Ma, Jiaying Zhu, Xiuren Zhang
      Pages 225-254

    4. Flow Cytometry and Sorting in Arabidopsis

      • David W. Galbraith, Guiling Sun
      Pages 255-294

    5. Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development

      • Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno, Pia Sappl, Marcus G. Heisler
      Pages 295-302

    6. Using Genetically Encoded Fluorescent Biosensors for Quantitative In Vivo Imaging

      • Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist, Heather N. Cartwright, David A. Quint, David W. Ehrhardt et al.
      Pages 303-322

    7. Using Tripartite Split-sfGFP for the Study of Membrane Protein–Protein Interactions

      • Tzu-Yin Liu
      Pages 323-336

    8. High-Pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-Labeling

      • Marisa S. Otegui
      Pages 337-347
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Keywords

About this book

This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Centro Nacional de Biotecnología, CSIC Centro Nacional de Biotecnología, Madrid, Spain

    Jose J. Sanchez-Serrano

  • Centro de Investigaciones Biologicas, CSIC Madrid Centro de Investigaciones Biologicas, Madrid, Spain

    Julio Salinas

Bibliographic Information

  • Book Title: Arabidopsis Protocols

  • Editors: Jose J. Sanchez-Serrano, Julio Salinas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0880-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0879-1Published: 12 November 2020

  • Softcover ISBN: 978-1-0716-0882-1Published: 12 November 2021

  • eBook ISBN: 978-1-0716-0880-7Published: 11 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XII, 465

  • Number of Illustrations: 1 b/w illustrations, 15 illustrations in colour

  • Topics: Plant Sciences

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