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Periodontal Pathogens

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Keiji Nagano

    1. Division of Microbiology, Department of Oral Biology School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan

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  2. Yoshiaki Hasegawa

    1. Department of Microbiology School of Dentistry, Aichi Gakuin University, Nisshin, Japan

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2210)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
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  2. Methods for Bacterial Genetic Manipulation

    1. Front Matter

      Pages 1-1
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    2. Site-Directed and Random Mutagenesis in Porphyromonas gingivalis: Construction of Fimbriae-Related-Gene Mutant

      • So-ichiro Nishiyama, Yoshiaki Hasegawa, Keiji Nagano
      Pages 3-14

    3. Genetic Manipulations of Oral Spirochete Treponema denticola

      • Kurni Kurniyati, Chunhao Li
      Pages 15-23

    4. Construction of a Gene-Deletion Mutant in Tannerella forsythia

      • Keiji Nagano, Yoshiaki Hasegawa
      Pages 25-31

    5. Construction of a Mutant in Prevotella melaninogenica Using the Conjugation Transfer Method with Escherichia coli

      • Yoshio Kondo
      Pages 33-41

    6. Genetic Transformation of Fusobacterium nucleatum

      • Akihiro Yoshida, Akihiko Ikegami
      Pages 43-50

  3. Experimental Methods to Examine Virulence Factors

    1. Front Matter

      Pages 51-51
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    2. Genotyping of Porphyromonas gingivalis in Relationship to Virulence

      • Atsuo Amano, Youn-Hee Choi, Hiroki Takeuchi
      Pages 53-59

    3. Transport and Polymerization of Porphyromonas gingivalis Type V Pili

      • Mikio Shoji, Satoshi Shibata, Mariko Naito, Koji Nakayama
      Pages 61-73

    4. Purification of Native Mfa1 Fimbriae from Porphyromonas gingivalis

      • Yoshiaki Hasegawa, Keiji Nagano, Yukitaka Murakami, Richard J. Lamont
      Pages 75-86

    5. Crystallization of Recombinant Fimbrial Proteins of Porphyromonas gingivalis

      • Thomas Heidler, Karina Persson
      Pages 87-96

    6. Enzymatic Characteristics and Activities of Gingipains from Porphyromonas gingivalis

      • Tomoko Kadowaki
      Pages 97-112

    7. Structural Characterization of the Type IX Secretion System in Porphyromonas gingivalis

      • Dhana G. Gorasia, Eric Hanssen, Paul D. Veith, Eric C. Reynolds
      Pages 113-121

    8. Methods for Functional Characterization of the Type IX Secretion System of Porphyromonas gingivalis

      • Keiko Sato
      Pages 123-133

    9. Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins

      • Sreedevi Chinthamani, Prasad R. Settem, Kiyonobu Honma, Takuma Nakajima, Ashu Sharma
      Pages 135-142

    10. Separation of Glycosylated OmpA-Like Proteins from Porphyromonas gingivalis and Tannerella forsythia

      • Yukitaka Murakami, Keiji Nagano, Yoshiaki Hasegawa
      Pages 143-155

    11. Intranasal Vaccine Study Using Porphyromonas gingivalis Membrane Vesicles: Isolation Method and Application to a Mouse Model

      • Satoru Hirayama, Ryoma Nakao
      Pages 157-166

    12. Analysis of the Butyrate-Producing Pathway in Porphyromonas gingivalis

      • Yasuo Yoshida
      Pages 167-172

    13. Characterization of the Treponema denticola Virulence Factor Dentilisin

      • Yuichiro Kikuchi, Kazuyuki Ishihara
      Pages 173-184
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Keywords

About this book

This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp. Beginning with methods for bacterial genetic manipulation, the volume continues with sections on experimental methods to examine virulence factors, interactions with other pathogenic microorganism and host cells, as well as a chapter on an animal model of periodontitis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Periodontal Pathogens: Methods and Protocols serves as an extensive and useful reference for researchers studying periodontal pathogens and will help elucidate the causes of periodontal disease and the systemic diseases related to it.

Editors and Affiliations

  • Division of Microbiology, Department of Oral Biology School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan

    Keiji Nagano

  • Department of Microbiology School of Dentistry, Aichi Gakuin University, Nisshin, Japan

    Yoshiaki Hasegawa

Bibliographic Information

  • Book Title: Periodontal Pathogens

  • Book Subtitle: Methods and Protocols

  • Editors: Keiji Nagano, Yoshiaki Hasegawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0939-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0938-5Published: 20 August 2020

  • Softcover ISBN: 978-1-0716-0941-5Published: 20 August 2021

  • eBook ISBN: 978-1-0716-0939-2Published: 20 August 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 256

  • Number of Illustrations: 27 b/w illustrations, 31 illustrations in colour

  • Topics: Microbiology, Dentistry, Biomedicine, general

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