Methods in Molecular Biology

Listeria Monocytogenes

Methods and Protocols

Editors: Fox, Edward M., Bierne, Hélène, Stessl, Beatrix (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-0716-0982-8
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About this book

This updated book explores a wide repertoire of tools and approaches that have been created, modified, and applied to the study of L. monocytogenes, forming the basis of our understanding of the bacterium today. Many of these key experimental techniques are gathered together herein. The volume presents aspects such as clinical disease and host-pathogen interactions, as well as the study of biofilms which present a significant challenge for control of the organism in the food processing environment. The topics covered in this edition also include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and methods for control of the organism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and up-to-date, Listeria monocytogenes: Methods and Protocols, Second Edition aims to contribute toward the harmonization of methods used to study this important bacterium, and to be of particular interest to Listeria research both in relation to food association and control as well as clinical microbiology.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Traditional Methods of Analysis for Listeria monocytogenes

    Pages 3-16

    Magalhães, Rui (et al.)

  • MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes

    Pages 17-29

    Jadhav, Snehal R. (et al.)

  • Sample Preparation for qPCR Detection of Listeria from Food

    Pages 31-40

    Mester, Patrick (et al.)

  • qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay

    Pages 41-53

    Witte, Anna Kristina (et al.)

  • Serotype Assignment by Sero-agglutination, ELISA, and PCR

    Pages 57-78

    Gorski, Lisa

Buy this book

eBook $149.00
price for USA
  • ISBN 978-1-0716-0982-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA

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Bibliographic Information

Bibliographic Information
Book Title
Listeria Monocytogenes
Book Subtitle
Methods and Protocols
Editors
  • Edward M. Fox
  • Hélène Bierne
  • Beatrix Stessl
Series Title
Methods in Molecular Biology
Series Volume
2220
Copyright
2021
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0982-8
DOI
10.1007/978-1-0716-0982-8
Hardcover ISBN
978-1-0716-0981-1
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 260
Number of Illustrations
24 b/w illustrations, 30 illustrations in colour
Topics