Skip to main content
  • Book
  • © 2021

Animal Models of Allergic Disease

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2223)

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. Applications of Mouse Models to the Study of Food Allergy

    • Sara Benedé, M. Cecilia Berin
    Pages 1-17
  3. A Mouse Model of Oral Sensitization to Hen’s Egg White

    • Sara Benedé, Daniel Lozano-Ojalvo, Rosina López-Fandiño, Elena Molina
    Pages 49-65
  4. Diet-Induced Mouse Model of Atopic Dermatitis

    • Masanori Fujii, Yuki Shimazaki, Takeshi Nabe
    Pages 79-86
  5. Experimental Mouse Models of Ragweed- and Papain-Induced Allergic Conjunctivitis

    • Akira Matsuda, Toshiaki Hirakata, Yosuke Asada, Susumu Nakae
    Pages 133-149
  6. Intravital Imaging of Vascular Permeability by Two-Photon Microscopy

    • Gyohei Egawa, Sachiko Ono, Kenji Kabashima
    Pages 151-157
  7. Isotype-Specific Detection of Serum Immunoglobulins Against Allergens

    • Danielle L. Germundson, Kumi Nagamoto-Combs
    Pages 159-167
  8. An Overview of Flow Cytometry: Its Principles and Applications in Allergic Disease Research

    • Taylor Schmit, Mitchell Klomp, M. Nadeem Khan
    Pages 169-182
  9. Cellular and Biochemical Analysis of Bronchoalveolar Lavage Fluid from Murine Lungs

    • Rama Satyanarayana Raju Kalidhindi, Nilesh Sudhakar Ambhore, Venkatachalem Sathish
    Pages 201-215
  10. Procedures to Evaluate Inflammatory and Pathological Changes During Allergic Airway Inflammation

    • Savita P. Rao, Stephanie Rastle-Simpson, Mythili Dileepan, P. Sriramarao
    Pages 217-236
  11. Assessment of Lung Eosinophils In Situ Using Immunohistological Staining

    • Christopher D. Nazaroff, William E. LeSuer, Mia Y. Masuda, Grace Pyon, Paige Lacy, Elizabeth A. Jacobsen
    Pages 237-266
  12. Preservation and Processing of Intestinal Tissue for the Assessment of Histopathology

    • Juliane Rieger, Lisa-Marie Pelckmann, Barbara Drewes
    Pages 267-280
  13. Antibiotic Treatment in an Animal Model of Inflammatory Lung Disease

    • Alissa Cait, Melina Messing, Jessica Cait, Diana Canals Hernaez, Kelly M. McNagny
    Pages 281-293

About this book

This volume provides protocols for mouse models of allergic diseases and guidelines for choosing a particular strains, allergen, adjuvant, and route of sensitization. Chapters detail types of allergic disease, methods that are frequently employed to analyze pathophysiology of allergic diseases, manipulation of intestinal microbiota, and desensitization of immure responses in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Animal Models of Allergic Disease: Methods and Protocols aims to offer a comprehensive collection of protocols and experience-derived instructions to further allergic disease research.

Editors and Affiliations

  • Department of Biomedical Sciences, University of North Dakota School of Medicine & Health Sciences, Grand Forks, USA

    Kumi Nagamoto-Combs

Bibliographic Information

  • Book Title: Animal Models of Allergic Disease

  • Book Subtitle: Methods and Protocols

  • Editors: Kumi Nagamoto-Combs

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1001-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1000-8Published: 24 November 2020

  • Softcover ISBN: 978-1-0716-1003-9Published: 24 November 2020

  • eBook ISBN: 978-1-0716-1001-5Published: 23 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 361

  • Number of Illustrations: 23 b/w illustrations, 75 illustrations in colour

  • Topics: Allergology, Molecular Medicine

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access