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Enzyme Kinetics in Drug Metabolism

Fundamentals and Applications

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Swati Nagar

    1. Department of Pharmaceutical Sciences, Temple University School of Pharmacy, Philadelphia, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Upendra A. Argikar

    1. Translational Medicine, Novartis Institutes for Biomedical Research, Inc., Cambridge, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Donald Tweedie

    1. Independent, Harleysville, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2342)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xxvii
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  2. Underlying Principles of Enzyme Kinetics

    1. Front Matter

      Pages 1-1
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    2. Fundamentals of Enzyme Kinetics: Michaelis-Menten and Non-Michaelis–Type (Atypical) Enzyme Kinetics

      • Eleanore Seibert, Timothy S. Tracy
      Pages 3-27

    3. Reversible Mechanisms of Enzyme Inhibition and Resulting Clinical Significance

      • Barbara Ring, Steven A. Wrighton, Michael Mohutsky
      Pages 29-50

    4. Irreversible Enzyme Inhibition Kinetics and Drug–Drug Interactions

      • Michael Mohutsky, Stephen D. Hall
      Pages 51-88

    5. Multienzyme Kinetics and Sequential Metabolism

      • Larry C. Wienkers, Brooke M. Rock
      Pages 89-112

    6. Consideration of the Unbound Drug Concentration in Enzyme Kinetics

      • Nigel J. Waters, R. Scott Obach, Li Di
      Pages 113-145

    7. Numerical Methods for Modeling Enzyme Kinetics

      • Jaydeep Yadav, Ken Korzekwa, Swati Nagar
      Pages 147-168

  3. Crystal Structure, Biochemistry, and Impact on Enzyme Kinetics

    1. Front Matter

      Pages 169-169
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    2. Crystal Structures of Drug-Metabolizing CYPs

      • D. Fernando Estrada, Amit Kumar, Christopher S. Campomizzi, Natalie Jay
      Pages 171-192

    3. The Structure and Mechanism of Drug Transporters

      • Arthur G. Roberts
      Pages 193-234

  4. Kinetics of Drug Metabolizing Enzymes and Transporters

    1. Front Matter

      Pages 235-235
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    2. Enzyme Kinetics of Oxidative Metabolism—Cytochromes P450

      • Ken Korzekwa
      Pages 237-256

    3. Enzyme Kinetics, Pharmacokinetics, and Inhibition of Aldehyde Oxidase

      • Erickson M. Paragas, Kanika Choughule, Jeffrey P. Jones, John T. Barr
      Pages 257-284

    4. Enzyme Kinetics of PAPS-Sulfotransferase

      • Margaret O. James
      Pages 285-300

    5. Enzyme Kinetics of Uridine Diphosphate Glucuronosyltransferases (UGTs)

      • Jin Zhou, Upendra A. Argikar, John O. Miners
      Pages 301-338

    6. Principles and Experimental Considerations for In Vitro Transporter Interaction Assays

      • Sid Bhoopathy, Chris Bode, Vatsala Naageshwaran, Erica Weiskircher-Hildebrandt, Venkata Mukkavilli, Ismael J. Hidalgo
      Pages 339-365

  5. Modeling Considerations

    1. Front Matter

      Pages 367-367
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    2. Prediction of Drug Clearance from Enzyme and Transporter Kinetics

      • Priyanka R. Kulkarni, Amir S. Youssef, Aneesh A. Argikar
      Pages 369-417

    3. Systems Biology Approaches to Enzyme Kinetics

      • Nnenna A. Finn, Andrew D. Raddatz, Melissa L. Kemp
      Pages 419-440
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Keywords

About this book

This second edition further develops the principles of applying kinetic principles to drug metabolizing enzymes and transporters. Chapters are divided into six sections detailing fundamental principles of enzyme kinetics, enzyme and transporter structures, highlighting specific oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for drug metabolizing enzymes and transporters, understanding of variability both experimental and interindividual (pharmacogenomic), and expanded case studies that provide real life examples of applying these principles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, in some cases step-by-step instructions with readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. 

 

Authoritativeand fully updated, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications, Second Edition serves as a practical teaching tool for novice and advanced scientists interested in the fundamental concepts.


Editors and Affiliations

  • Department of Pharmaceutical Sciences, Temple University School of Pharmacy, Philadelphia, USA

    Swati Nagar

  • Translational Medicine, Novartis Institutes for Biomedical Research, Inc., Cambridge, USA

    Upendra A. Argikar

  • Independent, Harleysville, USA

    Donald Tweedie

Bibliographic Information

  • Book Title: Enzyme Kinetics in Drug Metabolism

  • Book Subtitle: Fundamentals and Applications

  • Editors: Swati Nagar, Upendra A. Argikar, Donald Tweedie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1554-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1553-9Published: 17 July 2021

  • Softcover ISBN: 978-1-0716-1556-0Published: 18 July 2022

  • eBook ISBN: 978-1-0716-1554-6Published: 16 July 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XXVII, 881

  • Number of Illustrations: 29 b/w illustrations, 129 illustrations in colour

  • Topics: Pharmaceutical Sciences/Technology, Enzymology

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