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Glycosylation

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Gavin P. Davey

    1. School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2370)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Analytical and Bioinformatics Methods for Studying Glycosylation

    1. Front Matter

      Pages 1-1
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    2. Glycoinformatics Tools for Comprehensive Characterization of Glycans Enzymatically Released from Proteins

      • Ian Walsh, Sophie Zhao, Katherine Wongtrakul-Kish, Matthew Choo, Shi Jie Tay, Christopher H. Taron et al.
      Pages 3-23

    3. GlycoStore: A Platform for H/UPLC and Capillary Electrophoresis Glycan Data

      • Matthew P. Campbell, Sophie Zhao, Jodie L. Abrahams, Terry Nguyen-Khuong, Pauline M. Rudd
      Pages 25-40

    4. An Interactive View of Glycosylation

      • Julien Mariethoz, Davide Alocci, Niclas G. Karlsson, Nicolle H. Packer, Frédérique Lisacek
      Pages 41-65

    5. Characterization and Analysis of Food-Sourced Carbohydrates

      • Leonie J. Kiely, Rita M. Hickey
      Pages 67-95

    6. Comparative Glycomics Analysis of Mass Spectrometry Data

      • Yusen Zhou, Sriram Neelamegham
      Pages 97-113

  3. Synthetic Biology and Glycoengineering Methods for Controlling Glycosylation

    1. Front Matter

      Pages 115-115
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    2. Cell Free Remodeling of Glycosylation of Antibodies

      • Letícia Martins Mota, Venkata S. Tayi, Michael Butler
      Pages 117-146

    3. Rapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics

      • Pavlos Kotidis, Masue Marbiah, Roberto Donini, Itzcóatl A. Gómez, Ioscani Jimenez del Val, Stuart M. Haslam et al.
      Pages 147-167

    4. In Silico Analysis of Therapeutic Antibody Aggregation and the Influence of Glycosylation

      • Hyesoo Jeon, Jerrard M. Hayes, K. H. Mok
      Pages 169-183

    5. Recent Advances Toward Engineering Glycoproteins Using Modified Yeast Display Platforms

      • Anjali Shenoy, Adam W. Barb
      Pages 185-205

  4. Systems Biology and Computational Methods for Deciphering Glycosylation Networks

    1. Front Matter

      Pages 207-207
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    2. Computational Modeling of Glycan Processing in the Golgi for Investigating Changes in the Arrangements of Biosynthetic Enzymes

      • Ben West, A. Jamie Wood, Daniel Ungar
      Pages 209-222

    3. O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks

      • Andrew G. McDonald, Gavin P. Davey
      Pages 223-236

    4. Synthetic Strategies for FRET-Enabled Carbohydrate Active Enzyme Probes

      • Meenakshi Singh, Michael Watkinson, Eoin M. Scanlan, Gavin J. Miller
      Pages 237-264

  5. Glycosylation Biomarkers and Clinical Datasets

    1. Front Matter

      Pages 265-265
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    2. N-glycan Characterization by Liquid Chromatography Coupled with Fluorimetry and Mass Spectrometry

      • Richard A. Gardner, Paulina A. Urbanowicz, Daniel I. R. Spencer
      Pages 267-280

    3. Lectin Histochemistry for Tissues and Cells, and Dual Lectin and Antibody Co-localization

      • Siobhán S. McMahon, Michelle Kilcoyne
      Pages 281-299

    4. Lectin Affinity Chromatography for the Discovery of Novel Cancer Glycobiomarkers: A Case Study with PSA Glycoforms and Prostate Cancer

      • Esther Llop, Rosa Peracaula
      Pages 301-313
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Keywords

About this book

This volume explores the latest techniques and methods used for performing up-to-date glycosylation research. The chapters in this book are organized into four parts. Part One looks at the latest analytical and bioinformatics technologies that enable the characterization of glycosylation complexity. Part Two details the importance of synthetic chemistry and glycoengineering in the fields of bioprocessing and biotherapeutic development. Part Three discusses systems biology and computational technologies used by scientists to analyze glycosylation events in the cell. Part Four focuses on how cellular glycosylation biomarkers can be identified and used to characterize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Cutting-edge and practical, Glycosylation: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. 


Editors and Affiliations

  • School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland

    Gavin P. Davey

Bibliographic Information

  • Book Title: Glycosylation

  • Book Subtitle: Methods and Protocols

  • Editors: Gavin P. Davey

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1685-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1684-0Published: 07 October 2021

  • Softcover ISBN: 978-1-0716-1687-1Published: 07 October 2022

  • eBook ISBN: 978-1-0716-1685-7Published: 06 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 332

  • Number of Illustrations: 113 illustrations in colour

  • Topics: Biotechnology, Biochemistry, general, Bioinformatics

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