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Organ-on-a-Chip

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Marco Rasponi

    1. Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano, Italy

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible result
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2373)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Photo and Soft Lithography for Organ-on-Chip Applications

    • Erika Ferrari, Federico Nebuloni, Marco Rasponi, Paola Occhetta
    Pages 1-19

  3. Organ-on-Chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices

    • Mattia Ballerini, Mohammad Jouybar, Andrea Mainardi, Marco Rasponi, Giovanni Stefano Ugolini
    Pages 21-38

  4. Thermoplastic Microfluidics

    • Per Magnus Kristiansen, Agnieszka Karpik, Jerome Werder, Marco Guilherme, Michael Grob
    Pages 39-55

  5. Bile Duct-on-a-Chip

    • Yu Du, William J. Polacheck, Rebecca G. Wells
    Pages 57-68

  6. Establishment of a Modular Anaerobic Human Intestine Chip

    • Sasan Jalili-Firoozinezhad, Amir Bein, Francesca S. Gazzaniga, Cicely W. Fadel, Richard Novak, Donald E. Ingber
    Pages 69-85

  7. Intestinal Epithelium Tubules on a Chip

    • Kinga Kosim, Iris Schilt, Henriëtte L. Lanz, Paul Vulto, Dorota Kurek
    Pages 87-105

  8. A Human Neurovascular Unit On-a-Chip

    • Sharon Wei Ling Lee, Renato Rogosic, Claudia Venturi, Manuela Teresa Raimondi, Andrea Pavesi, Giulia Adriani
    Pages 107-119

  9. Generation of a Glomerular Filtration Barrier on a Glomerulus-on-a-Chip Platform

    • Laura Perin, Stefano Da Sacco
    Pages 121-131

  10. Electromechanical Stimulation of 3D Cardiac Microtissues in a Heart-on-Chip Model

    • Roberta Visone, Paola Occhetta, Marco Rasponi
    Pages 133-157

  11. Miniaturized Vascularized Bleeding Model of Hemostasis

    • Elaissa Trybus Hardy, Yumiko Sakurai, Wilbur A. Lam
    Pages 159-175

  12. Fabrication and Use of a Pumpless Microfluidic Lymphatic Vessel Chip

    • Parinaz Fathi, Mandy B. Esch
    Pages 177-199

  13. On-Chip Platelet Activation Assessment: Microfluidic Emulation of Shear Stress Profiles Induced by Mechanical Circulatory Support Devices

    • Tatiana Mencarini, Silvia Bozzi, Alberto Redaelli
    Pages 201-212

  14. Biofabrication of 3D Human Muscle Model with Vascularization and Endomysium

    • Simone Bersini, Riccardo Francescato, Matteo Moretti
    Pages 213-230

  15. Mechanical Induction of Osteoarthritis Traits in a Cartilage-on-a-Chip Model

    • Andrea Mainardi, Paola Occhetta, Marco Rasponi
    Pages 231-251

  16. Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation

    • Silvia Palombella, Shima Salehi, Carlotta Mondadori, Giuseppe Talò, Valerio Sansone, Matteo Moretti et al.
    Pages 253-266

  17. A Mesoscale 3D Culture System for Native and Engineered Biphasic Tissues: Application to the Osteochondral Unit

    • Irene Chiesa, Roberto Di Gesù, Kalon J. Overholt, Riccardo Gottardi
    Pages 267-281

  18. 3D Biofabricated In Vitro Models of Vascularized and Mineralized Bone Tissues

    • Maria Vittoria Colombo, Simone Bersini, Chiara Arrigoni, Matteo Moretti
    Pages 283-296

  19. Isolation, Integration, and Culture of Human Mature Adipocytes Leveraging Organ-on-Chip Technology

    • Julia Rogal, Julia Roosz, Peter Loskill
    Pages 297-313

  20. Back Matter

    Pages 315-322
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Keywords

About this book

This book provides a collection of microphysiological systems employed for chemical/drug screening and strategies to mimic various physiological conditions. Chapters guide readers through Organ-on-a-Chip( OoC) platforms such as liver, intestine, blood-brain barrier, kidney, vessels, cardiac and skeletal muscles, articular joint, human fat. Additional chapters detail microfabrication technologies used to fabricate OoC devices such as, standard photo- and soft-lithography, techniques to fabricate membranes, and industrial-oriented fabrication methods.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Organ-On-a Chip: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Editors and Affiliations

  • Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano, Italy

    Marco Rasponi

Bibliographic Information

  • Book Title: Organ-on-a-Chip

  • Book Subtitle: Methods and Protocols

  • Editors: Marco Rasponi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1693-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1692-5Published: 15 September 2021

  • Softcover ISBN: 978-1-0716-1695-6Published: 16 September 2022

  • eBook ISBN: 978-1-0716-1693-2Published: 14 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 322

  • Number of Illustrations: 12 b/w illustrations, 71 illustrations in colour

  • Topics: Biotechnology, Pharmacology/Toxicology

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