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Glycan Microarrays

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Michelle Kilcoyne

    1. Carbohydrate Signalling Group, Discipline of Microbiology School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

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  2. Jared Q. Gerlach

    1. Advanced Glycoscience Research Cluster, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2460)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
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  3. Glycan Microarray Data Extraction, Processing and use for Measurements

    1. Front Matter

      Pages 65-65
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    2. General Strategies for Glycan Microarray Data Processing and Analysis

      • J. Sebastian Temme, Jeffrey C. Gildersleeve
      Pages 67-87

    3. Calculating Half Maximal Inhibitory Concentration (IC50) Values from Glycomics Microarray Data Using GraphPad Prism

      • Marie Le Berre, Jared Q. Gerlach, Iwona DziembaÅ‚a, Michelle Kilcoyne
      Pages 89-111

  4. Complex Natural and Synthetic Glycoconjugate Microarrays

    1. Front Matter

      Pages 113-113
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    2. Synthetic Plant Glycan Microarrays as Tools for Plant Biology

      • Colin Ruprecht, Fabian Pfrengle
      Pages 115-125

    3. Mucin Purification and Printing Natural Mucin Microarrays

      • Marie Le Berre, Jared Q. Gerlach, Mary E. Gallagher, Lokesh Joshi, Stephen D. Carrington, Michelle Kilcoyne
      Pages 127-146

    4. Bacterial Microarrays for Examining Bacterial Glycosignatures and Recognition by Host Lectins

      • María Asunción Campanero-Rhodes, Dolores Solís
      Pages 147-160

    5. Tissue Glycome Mapping: Lectin Microarray-Based Differential Glycomic Analysis of Formalin-Fixed Paraffin-Embedded Tissue Sections

      • Chiaki Nagai-Okatani, Xia Zou, Atsushi Matsuda, Yoko Itakura, Masashi Toyoda, Yan Zhang et al.
      Pages 161-180

  5. Applications for Glycomics Microarrays

    1. Front Matter

      Pages 181-181
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    2. Detection of Autoantibodies Using Combinatorial Glycolipid Microarrays

      • Susan K. Halstead, Dawn Gourlay, Hugh J. Willison
      Pages 183-191

    3. Glycan Microarrays Containing Synthetic Streptococcus pneumoniae CPS Fragments and Their Application to Vaccine Development

      • Paulina Kaplonek, Peter H. Seeberger
      Pages 193-206

    4. Lectin-Based Protein Microarray for the Glycan Analysis of Colorectal Cancer Biomarkers: The Insulin-Like Growth Factor System

      • Dragana Robajac, Martina Križáková, MiloÅ¡ Å underić, Goran MiljuÅ¡, Peter Gemeiner, Olgica Nedić et al.
      Pages 207-222

    5. Bacterial Staining and Profiling for Glycan Interactions on Glycan Microarrays for t-Test Calculation

      • Stephen Cunningham, Jared Q. Gerlach, Andrea Flannery, Michelle Kilcoyne
      Pages 223-237

    6. Preparation and Fluorescent Labeling of Cell-Derived Micelles and Profiling on Glycan Microarrays

      • Marie Le Berre, Jared Q. Gerlach, Michelle Kilcoyne
      Pages 239-248
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Keywords

About this book

This volume encompasses short targeted reviews and methods featuring the construction and use of glycan microarray platforms. Chapters guider readers through glycan microarrays comprised of synthetic glycans, natural biomolecules, bacteria, neoglycoproteins,  various applications and progress of these platforms. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform.

Editors and Affiliations

  • Carbohydrate Signalling Group, Discipline of Microbiology School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Michelle Kilcoyne

  • Advanced Glycoscience Research Cluster, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Jared Q. Gerlach

Bibliographic Information

  • Book Title: Glycan Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Michelle Kilcoyne, Jared Q. Gerlach

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2148-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2147-9Published: 23 December 2021

  • Softcover ISBN: 978-1-0716-2150-9Published: 23 December 2021

  • eBook ISBN: 978-1-0716-2148-6Published: 01 January 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 253

  • Number of Illustrations: 7 b/w illustrations, 65 illustrations in colour

  • Topics: Biochemistry, general

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