Modern Methods in Analytical Morphology

1. Front Matter

   Pages i-xii

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2. The Use of Silver Stains in the Identification of Neuroendocrine Cell Types

   • Lars Grimelius, Huici Su, Gerhard W. Hacker

   Pages 1-8

3. Quantitative Immunohistochemistry for the Investigation of Regulatory Peptides in Health and Disease

   • Giorgio Terenghi, Julia M. Polak

   Pages 9-18

4. Immunogold-Silver Staining (IGSS) for Detection of Antigenic Sites and DNA Sequences

   • Gerhard W. Hacker, Cornelia Hauser-Kronberger, Anton-Helmut Graf, Gorm Danscher, Jiang Gu, Lars Grimelius

   Pages 19-35

5. Single and Multiple Immunofluorescence - An Overview

   • Cornelia Hauser-Kronberger, Gerhard W. Hacker

   Pages 37-50

6. Immunocytochemistry of Whole-Mount Preparations

   • Gian-Luca Ferri, Rosa Maria Gaudio

   Pages 51-66

7. Microwave Immunocytochemistry

   • Jiang Gu

   Pages 67-80

8. Basic Principles of Immunohistopathology

   • Gerhard W. Hacker, Anton-Helmut Graf, Otto Dietze

   Pages 81-112

9. Classification of Endocrine Tumors by Histochemical Techniques

   • Erik Wilander

   Pages 113-120

10. Modern Methods in Cytology

    • Siew-Khin Tang, Anthony S.-Y. Leong

    Pages 121-155

11. The Use of the Multiblock in Diagnostic Immunohistochemistry

    • Wolfgang Kraaz, Lena Scheibenpflug

    Pages 157-161

12. Immunohistochemical, Biochemical and Physiological Characterization of Calcium-Binding Proteins

    • A. Hermann, H. H. Kerschbaum

    Pages 163-185

13. Pre-Embedding Immunocytochemistry in Transmission Electron Microscopy

    • Wolfgang Kummer, Cornelia Hauser-Kronberger, Wolfgang H. Muss

    Pages 187-201

14. Post-Embedding Localization Methods for Electron Microscopy: Basic Approach and Protocols

    • Peter M. Lackie, Jürgen Roth

    Pages 203-224

15. Immunogold-Silver Staining (IGSS) for Immunoelectron Microscopy and in Multiple Detection Affinity Cytochemistry

    • Tibor Krenács, Lászlá Krenács

    Pages 225-251

16. Postembedding Light and Electron Microscopic Immunocytochemistry in Pineal Photoneuroendocrinology

    • Ingeborg Vigh-Teichmann, Bela Vigh

    Pages 253-270

17. In Situ Hybridization for Peptide mRNA

    • Giorgio Terenghi, Julia M. Polak

    Pages 271-282

18. Principles of the Polymerase Chain Reaction

    • Erich Arrer, Gerhard W. Hacker, Angelika Schiechl, Jiang Gu

    Pages 283-295

19. Polymerase Chain Reaction (PCR) In Situ Hybridization: Detection of Human Papillomavirus (HPV) DNA in SiHa Cell Monolayers

    • Ingeborg Zehbe, Jan Sällström, Erik Wilander, Gerhard W. Hacker, Eva Rylander, Anders Strand et al.

    Pages 297-306

20. Axonal Transport Tracing Combined with Neurotransmitter and Peptide Immunocytochemistry

    • Huici Su, Jiang Gu, Gerhard W. Hacker

    Pages 307-325

While advances in modem medicine largely parallel our understanding of morphology, discoveries in morphology are propelled by developments of new tools and means to visualize and measure tissue elements. The invention of dissecting, light, fluorescence and electron microscopes together with advances in labeling and staining techniques are among the stepping stones of morphological progress. Today, we are in an exciting new era when classical morphology is being combined with developments from other disciplines. The combination of morphology and immunology resulted in immunocytochemistry; morphology and molecular biology led to in situ hybridization and in situ PCR. Adding computer science to morphology gave birth to image analysis. Combining laser technology and the microsope evolved into confocal microscope. For more than a decade, modem morphology has continued to develop by merging with other disciplines at a rate that is still gathering momentum, providing exciting and dynamic new frontiers for other biological fields. "Modem Methods in Analytical Morphology," based largely on the "First International Workshop on Modem Methods in Analytical Histochemistry, "is an updated review of the current trends in the field. It covers an extensive array of new technical developments in major disciplines of modem morphology. The authors are not only leaders in their fields but also have extensive "hands on" experience with "bench work. " Their chapters are written in a comprehensive manner including discussion of both theoretical considerations and practical applications to give the readers a broad view of the topics covered.

• biology
• chemistry
• classification
• development
• immunology
• morphology
• tissue

• Deborah Research Institute, Browns Mills, USA

  Jiang Gu

• Institute of Pathological Anatomy, Salzburg, Austria

  Gerhard W. Hacker

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