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Pseudomonas Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Alain Filloux

    1. MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Juan-Luis Ramos

    1. Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1149)

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Table of contents (61 protocols)

  1. Front Matter

    Pages i-xviii
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  2. Basic Pseudomonas Features

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Gene Transfer: Transduction

      • Emanuela Frangipani
      Pages 3-10

    3. Gene Transfer: Transformation/Electroporation

      • Frédéric Cadoret, Chantal Soscia, Romé Voulhoux
      Pages 11-15

    4. Gene Transfer: Conjugation

      • Thibault G. Sana, Aurélie Laubier, Sophie Bleves
      Pages 17-22

    5. Pseudomonas Bacteriophage Isolation and Production

      • Joana Azeredo, Sanna Sillankorva, Diana P. Pires
      Pages 23-32

    6. Genotyping Methods

      • Burkhard Tümmler
      Pages 33-47

    7. Drug Susceptibility Testing by Dilution Methods

      • Katy Jeannot, Patrick Plésiat
      Pages 49-58

    8. Plate-Based Assay for Swimming Motility in Pseudomonas aeruginosa

      • Dae-Gon Ha, Sherry L. Kuchma, George A. O’Toole
      Pages 59-65

    9. Plate-Based Assay for Swarming Motility in Pseudomonas aeruginosa

      • Dae-Gon Ha, Sherry L. Kuchma, George A. O’Toole
      Pages 67-72

    10. Motility Assay: Twitching Motility

      • Lynne Turnbull, Cynthia B. Whitchurch
      Pages 73-86

    11. Qualitative and Quantitative Assays for Flagellum-Mediated Chemotaxis

      • José Antonio Reyes Darias, Cristina García-Fontana, Andrés Corral Lugo, Miriam Rico-Jiménez, Tino Krell
      Pages 87-97

    12. Microscopic Analysis: Morphotypes and Cellular Appendages

      • Olga Zaborina, John Alverdy, Megha Shah, Yimei Chen
      Pages 99-107

  3. Proteins and Protein Transport

    1. Front Matter

      Pages 109-109
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    2. Determination of Lipolytic Enzyme Activities

      • Karl-Erich Jaeger, Filip Kovacic
      Pages 111-134

    3. Elastinolytic and Proteolytic Enzymes

      • Efrat Kessler, Mary Safrin
      Pages 135-169

    4. In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins

      • Stephanie L. Rolsma, Dara W. Frank
      Pages 171-184

    5. Cell Fractionation

      • Bérengère Ize, Véronique Viarre, Romé Voulhoux
      Pages 185-191

    6. Characterization of Molecular Interactions Using Isothermal Titration Calorimetry

      • Tino Krell, Jesús Lacal, Cristina García-Fontana, Hortencia Silva-Jiménez, Miriam Rico-Jiménez, Andrés Corral Lugo et al.
      Pages 193-203

    7. Proteomic Analysis

      • Pascal Cosette, Thierry Jouenne
      Pages 205-212
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Keywords

About this book

In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.

Editors and Affiliations

  • MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

    Alain Filloux

  • Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Juan-Luis Ramos

Bibliographic Information

  • Book Title: Pseudomonas Methods and Protocols

  • Editors: Alain Filloux, Juan-Luis Ramos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0473-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0472-3Published: 13 May 2014

  • Softcover ISBN: 978-1-4939-4416-3Published: 03 September 2016

  • eBook ISBN: 978-1-4939-0473-0Published: 12 May 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 816

  • Number of Illustrations: 68 b/w illustrations, 89 illustrations in colour

  • Topics: Microbiology, Bacteriology

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