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Innate DNA and RNA Recognition

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Hans-Joachim Anders

    1. Ludwig Maximilians Universität München, Medizinische Klinik und Poliklinik IV, München, Germany

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  2. Adriana Migliorini

    1. Helmholtz Zentrum München German Researc, Institute for Diabetes and Regeneration, Munich, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1169)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Analysis of Viral Nucleic Acid Sensing In-Silico and In-Vitro

    1. Front Matter

      Pages 1-1
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    2. Detection of RNA Modifications by HPLC Analysis and Competitive ELISA

      • Gernot Nees, Andreas Kaufmann, Stefan Bauer
      Pages 3-14

    3. Enzymatic Synthesis and Purification of a Defined RIG-I Ligand

      • Marion Goldeck, Martin Schlee, Gunther Hartmann, Veit Hornung
      Pages 15-25

    4. Crystallization of Mouse RIG-I ATPase Domain: In Situ Proteolysis

      • Filiz Civril, Karl-Peter Hopfner
      Pages 27-35

    5. Isolation of RIG-I-Associated RNAs from Virus-Infected Cells

      • Andreas Schmidt, Andreas Linder, Nicolas Linder, Simon Rothenfusser
      Pages 37-44

    6. Structure Modeling of Toll-Like Receptors

      • Jing Gong, Tiandi Wei
      Pages 45-53

    7. Nucleic Acid Recognition in Dendritic Cells

      • Alexander Heiseke, Katharina Eisenächer, Anne Krug
      Pages 55-65

    8. Viral Nucleic Acid Recognition in Human Nonimmune Cells: In Vitro Systems

      • Andrea Ribeiro, Markus Wörnle
      Pages 67-75

    9. Analysis of Nucleic Acid-Induced Nonimmune Cell Death In Vitro

      • Simone Romoli, Adriana Migliorini
      Pages 77-86

    10. In Vitro Analysis of Nucleic Acid Recognition in B Lymphocytes

      • Saskia Ziegler, Isabelle Bekeredjian-Ding
      Pages 87-96

    11. Mapping of Optimal CD8 T Cell Epitopes

      • Julia Roider, Thomas Vollbrecht, Rika Draenert
      Pages 97-106

    12. A Modular Approach to Suppression Assays: TLR Ligands, Conditioned Medium, and Viral Infection

      • Viktor H. Koelzer, David Anz
      Pages 107-120

    13. MicroRNA Methodology: Advances in miRNA Technologies

      • Theresa Kaeuferle, Sabine Bartel, Stefan Dehmel, Susanne Krauss-Etschmann
      Pages 121-130

  3. Analysis of Nucleic Acid Sensing In-Vivo

    1. Front Matter

      Pages 131-131
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    2. Expression Profiling by Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)

      • Maciej Lech, Hans-Joachim Anders
      Pages 133-142

    3. Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus

      • Amanda A. Watkins, Ramon G. B. Bonegio, Ian R. Rifkin
      Pages 143-158

    4. Induction and Analysis of Nephrotoxic Serum Nephritis in Mice

      • John M. Hoppe, Volker Vielhauer
      Pages 159-174

    5. Isolation of Intratumoral Leukocytes of TLR-Stimulated Tumor-Bearing Mice

      • Moritz Rapp, David Anz, Max Schnurr
      Pages 175-179

    6. Bifunctional siRNAs for Tumor Therapy

      • Fanny Matheis, Robert Besch
      Pages 181-192
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Keywords

About this book

Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.

Editors and Affiliations

  • Ludwig Maximilians Universität München, Medizinische Klinik und Poliklinik IV, München, Germany

    Hans-Joachim Anders

  • Helmholtz Zentrum München German Researc, Institute for Diabetes and Regeneration, Munich, Germany

    Adriana Migliorini

Bibliographic Information

  • Book Title: Innate DNA and RNA Recognition

  • Book Subtitle: Methods and Protocols

  • Editors: Hans-Joachim Anders, Adriana Migliorini

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0882-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0881-3Published: 24 June 2014

  • Softcover ISBN: 978-1-4939-4583-2Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0882-0Published: 23 June 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 195

  • Number of Illustrations: 21 b/w illustrations, 16 illustrations in colour

  • Topics: Human Genetics, Gene Function

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