Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1315)
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Table of contents (27 protocols)
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Front Matter
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Analysis of Genetic Variation
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Front Matter
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Analysis of DNA Methylation Patterns
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Front Matter
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About this book
The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology.
Editors and Affiliations
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Medizinische Hochschule Hannover, Institute of Pathology, Hannover, Germany
Ulrich Lehmann
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Laboratory for Epigenetics and Environment, Centre National de Génotypage, CEA- Institut de Génomique, Evry, France
Jörg Tost
Bibliographic Information
Book Title: Pyrosequencing
Book Subtitle: Methods and Protocols
Editors: Ulrich Lehmann, Jörg Tost
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2715-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2714-2Published: 24 June 2015
Softcover ISBN: 978-1-4939-4909-0Published: 09 October 2016
eBook ISBN: 978-1-4939-2715-9Published: 23 June 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 414
Number of Illustrations: 26 b/w illustrations, 57 illustrations in colour
Topics: Gene Function