Proteomics for Biomarker Discovery
Methods and Protocols
Editors: Brun, Virginie, Couté, Yohann (Eds.)
Free Preview- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains implementation advice from the experts
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- About this book
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This volume presents modern and enhanced methods that detail techniques to perform proteomics analyses dedicated to biomarker discovery for human health. Chapters guide readers through pre/post analytical factors, protocols for the preparation of extracellular vesicles and exosomes, and various analytical pipelines including Data Independent Acquisition (DIA), discovery, as well as targeted and top-down proteomics analysis workflows. Bioinformatics tools and workflows to select and evaluate candidate biomarkers or combinations of biomarkers are also presented. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Proteomics for Biomarker Discovery: Methods and Protocols aims to ensure successful results in the further study of this vital field.
- Table of contents (18 chapters)
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Pre- and Post-analytical Factors in Biomarker Discovery
Pages 1-22
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Pre-fractionation of Noncirculating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers
Pages 23-37
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Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers
Pages 39-50
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Protein Biomarker Discovery Using Human Blood Plasma Microparticles
Pages 51-64
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A Standardized and Reproducible Proteomics Protocol for Bottom-Up Quantitative Analysis of Protein Samples Using SP3 and Mass Spectrometry
Pages 65-87
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Table of contents (18 chapters)
Bibliographic Information
- Bibliographic Information
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- Book Title
- Proteomics for Biomarker Discovery
- Book Subtitle
- Methods and Protocols
- Editors
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- Virginie Brun
- Yohann Couté
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1959
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9164-8
- DOI
- 10.1007/978-1-4939-9164-8
- Hardcover ISBN
- 978-1-4939-9163-1
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XII, 292
- Number of Illustrations
- 5 b/w illustrations, 47 illustrations in colour
- Topics
