Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the expert
Part of the book series: Methods in Molecular Biology (MIMB, volume 2019)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
Keywords
About this book
This volume explores the latest synthetic procedures for producing receptor-specific retinoids and rexinoids, molecular biology methods, and new technologies to demonstrate the therapeutic activities of molecules. The chapters in this book cover topics such as lentiviral delivery of shRNA constructs into acute promyelocytic leukemia cells for ATRA induced differentiation and autophagy; methods to analyze RAR signaling in colorectal cancer cells; differentiation of primary myoblasts by using RXR agonist; methodology for analyzing effects of retinoid treatment on nervous system development and larval swimming behavior; and protocols for assessment of autophagic flux in ATRA treated 2D and 3D breast cancer cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshootingand avoiding known pitfalls.
Cutting-edge and comprehensive, Retinoid and Rexinoid Signaling: Methods and Protocols is a valuable resources for graduate students, postdoctoral fellows, and principal investigators who are interested in further exploring the signaling mechanisms of these molecules in their specific preclinical models.
Editors and Affiliations
Bibliographic Information
Book Title: Retinoid and Rexinoid Signaling
Book Subtitle: Methods and Protocols
Editors: Swapan K. Ray
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9585-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9584-4Published: 30 July 2019
Softcover ISBN: 978-1-4939-9587-5Published: 14 August 2020
eBook ISBN: 978-1-4939-9585-1Published: 29 July 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 276
Number of Illustrations: 28 b/w illustrations, 36 illustrations in colour
Topics: Biochemistry, general, Cancer Research