- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume provides a variety of methods used to analyze ADAMTS proteases and ADAMTS-like proteins, including their structure, substrate profile, tissue and cell distribution, post-translational modification and biological pathways. Chapters discuss genetic, cell biology, biochemical and proteomics techniques applicable to the analysis of all the members of the ADAMTS superfamily. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, ADAMTS Proteases: Methods and Protocols is a valuable tool for researchers to study both simple and sophisticated methodologies that are often difficult to locate in primary research literature.
- Table of contents (24 chapters)
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ADAMTS Proteins: Concepts, Challenges, and Prospects
Pages 1-12
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Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology
Pages 13-24
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Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays
Pages 25-43
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Challenges and Solutions for Purification of ADAMTS Proteases: An Overview
Pages 45-53
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Purification of Native or Recombinant ADAMTS2, and Procollagen I Cleavage Assay
Pages 55-62
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Table of contents (24 chapters)
Bibliographic Information
- Bibliographic Information
-
- Book Title
- ADAMTS Proteases
- Book Subtitle
- Methods and Protocols
- Editors
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- Suneel S. Apte
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2043
- Copyright
- 2020
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9698-8
- DOI
- 10.1007/978-1-4939-9698-8
- Hardcover ISBN
- 978-1-4939-9697-1
- Softcover ISBN
- 978-1-4939-9700-8
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 299
- Number of Illustrations
- 12 b/w illustrations, 47 illustrations in colour
- Topics
