Overview
- Editors:
-
-
Russell J. Diefenbach
-
Department of Biomedical Sciences, Macquarie University, Sydney, Australia
-
Cornel Fraefel
-
Institute of Virology, University of Zurich, Zürich, Switzerland
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Access this book
Other ways to access
Table of contents (27 protocols)
-
-
- Christopher E. Denes, Roger D. Everett, Russell J. Diefenbach
Pages 1-30
-
- Kerrie J. Sandgren, Naomi R. Truong, Jacinta B. Smith, Kirstie Bertram, Anthony L. Cunningham
Pages 31-56
-
- Sereina O. Sutter, Peggy Marconi, Anita F. Meier
Pages 57-72
-
- William F. Goins, Shaohua Huang, Bonnie Hall, Marco Marzulli, Justus B. Cohen, Joseph C. Glorioso
Pages 73-90
-
- Cornel Fraefel, Alberto L. Epstein
Pages 91-109
-
- Anita F. Meier, Andrea S. Laimbacher
Pages 111-130
-
- Laura Menotti, Valerio Leoni, Valentina Gatta, Biljana Petrovic, Andrea Vannini, Simona Pepe et al.
Pages 131-151
-
- Andrea Vannini, Biljana Petrovic, Valentina Gatta, Valerio Leoni, Simona Pepe, Laura Menotti et al.
Pages 153-168
-
- Thilaga Velusamy, Anjali Gowripalan, David C. Tscharke
Pages 169-183
-
- Camille Cohen, Armelle Corpet, Mohamed Ali Maroui, Franceline Juillard, Patrick Lomonte
Pages 185-197
-
- Mackenzie M. Shipley, Molly M. Rathbun, Moriah L. Szpara
Pages 199-217
-
- Nancy M. Sawtell, Richard L. Thompson
Pages 219-239
-
- Andreas Sauerbrei, Kathrin Bohn-Wippert
Pages 241-261
-
- Hui-Lan Hu, Kalanghad Puthankalam Srinivas, Ian Mohr, Tony T. Huang, Angus C. Wilson
Pages 263-277
-
-
- Bita Khadivjam, Nabil El Bilali, Roger Lippé
Pages 289-303
-
- Raquel Bello-Morales, José Antonio López-Guerrero
Pages 305-317
-
- Tri Komala Sari, Katrina A. Gianopulos, Anthony V. Nicola
Pages 319-326
-
- Mujeeb R. Cheerathodi, David G. Meckes Jr.
Pages 327-341
About this book
This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV.
Editors and Affiliations
-
Department of Biomedical Sciences, Macquarie University, Sydney, Australia
Russell J. Diefenbach
-
Institute of Virology, University of Zurich, Zürich, Switzerland
Cornel Fraefel
