Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2066)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (18 protocols)
-
Front Matter
-
Back Matter
About this book
This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field.
Editors and Affiliations
-
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Transgenic and Gene-Targeting Facility, University of Kansas Medical Center, Kansas City, KS, USA, Kansas City, USA
Melissa A. Larson
Bibliographic Information
Book Title: Transgenic Mouse
Book Subtitle: Methods and Protocols
Editors: Melissa A. Larson
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9837-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-4939-9836-4Published: 12 September 2019
Softcover ISBN: 978-1-4939-9839-5Published: 12 September 2020
eBook ISBN: 978-1-4939-9837-1Published: 11 September 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 236
Number of Illustrations: 18 b/w illustrations, 27 illustrations in colour
Topics: Human Genetics, Animal Models, Stem Cells