Overview
- Presents a detailed compendium of techniques used routinely in the leading laboratories utilizing current, common technologies, especially those established for proteomics research
- Reviews the current practices used for the analysis of the specific single O-GlcNAc found on nuclear and cytoplasmic proteins
- Disperses the misconception that only experts can analyze the glycome
- Inspires researchers to pursue glycan function through sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 534)
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Table of contents (26 protocols)
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Glycan Analysis
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Glycopeptides
Keywords
About this book
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
Reviews
Bibliographic Information
Book Title: Glycomics
Book Subtitle: Methods and Protocols
Editors: Niclas G. Karlsson, Nicolle H. Packer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-022-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-774-7Published: 01 April 2009
Softcover ISBN: 978-1-61737-769-3Published: 19 November 2010
eBook ISBN: 978-1-59745-022-5Published: 11 March 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 390
Topics: Biochemistry, general, Human Physiology, Carbohydrate Chemistry