Quorum Sensing

1. Front Matter

   Pages i-xvi

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2. Detection, Isolation, and Characterization of Quorum Sensing Compounds

   1. Front Matter

      Pages 1-1

      PDF

   2. Bioassays of Quorum Sensing Compounds Using Agrobacterium tumefaciens and Chromobacterium violaceum

      • Weihua Chu, Dhiraj A. Vattem, Vatsala Maitin, Mary B. Barnes, Robert J. C. McLean

      Pages 3-19

   3. Detection of 2-Alkyl-4-Quinolones Using Biosensors

      • Stephen P. Diggle, Matthew P. Fletcher, Miguel Cámara, Paul Williams

      Pages 21-30

   4. FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds

      • Sathish Rajamani, Richard Sayre

      Pages 31-46

   5. Isolation of agr Quorum Sensing Autoinducers

      • Naomi Balaban

      Pages 47-59

   6. Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of N-Acyl-l-Homoserine Lactones and 4-Hydroxy-2-Alkylquinolines

      • François Lépine, Eric Déziel

      Pages 61-69

   7. Detection of Autoinducer (AI-2)-Like Activity in Food Samples

      • Kirthiram K. Sivakumar, Palmy R. Jesudhasan, Suresh D. Pillai

      Pages 71-82

   8. Detection of Bacterial Signaling Molecules in Liquid or Gaseous Environments

      • Peter Edmonson, Desmond Stubbs, William Hunt

      Pages 83-100

   9. Determination of Acyl Homoserine Lactone and Tetramic Acid Concentrations in Biological Samples

      • Colin A. Lowery, Gunnar F. Kaufmann, Kim D. Janda

      Pages 101-111

   10. Luminescent Reporters and Their Applications for the Characterization of Signals and Signal-Mimics that Alter LasR-Mediated Quorum Sensing

       • Ali Alagely, Sathish Rajamani, Max Teplitski

       Pages 113-130

3. Determining the Function of Autoinducers In vivo

   1. Front Matter

      Pages 131-131

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   2. Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules

      • Vladimir V. Kravchenko, Richard J. Ulevitch, Gunnar F. Kaufmann

      Pages 133-145

   3. Imaging N-Acyl Homoserine Lactone Quorum Sensing In Vivo

      • Louise Dahl Christensen, Maria van Gennip, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt

      Pages 147-157

   4. Defining the Structure and Function of Acyl-Homoserine Lactone Autoinducers

      • Mair E. A. Churchill, Hiruy M. Sibhatu, Charis L. Uhlson

      Pages 159-171

   5. Global Expression Analysis of Quorum-Sensing Controlled Genes

      • Martin Schuster

      Pages 173-187

   6. Small RNA Target Genes and Regulatory Connections in the Vibrio cholerae Quorum Sensing System

      • Brian K. Hammer, Sine Lo Svenningsen

      Pages 189-206

   7. Quantifying Pseudomonas aeruginosa Quinolones and Examining Their Interactions with Lipids

      • Gregory C. Palmer, Jeffrey W. Schertzer, Lauren Mashburn-Warren, Marvin Whiteley

      Pages 207-217

   8. Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans

      • Aurélie Deveau, Deborah A. Hogan

      Pages 219-233

   9. Design of Synthetic Mammalian Quorum-Sensing Systems

      • Wilfried Weber, Martin Fussenegger

      Pages 235-249

Since its early days in the 1990s, the Quorum Sensing (QS) field has grown from a few dozen laboratories, investigating the pathways, proteins, and chemicals that facilitate signaling in bacteria, to hundreds of groups that have integrated evolutionary biology, computer science, mathematics, engineering, and metagenomics to create an ever-expanding and dynamic field. In Quorum Sensing: Methods and Protocols, expert researchers provide an in-depth set of diverse protocols that span this broad area of study. Broken into three detailed sections, the volume covers the detection, isolation, and characterization of the QS signals made by both Gram- and Gram+ bacteria, determination of the function of QS signals in vivo, and the development of QS disruption strategies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known experimental pitfalls. Comprehensive and cutting-edge, Quorum Sensing: Methods and Protocols serves as an invaluable collection of easily accessible techniques for scientists seeking to further our knowledge about bacterial communication and its relation to humanity.

• Antibiotic resistant infections
• Bacteria
• Biofilms
• Cell-to-cell signaling
• Communication systems
• Gram- and Gram+
• QS disruption
• QS signals

• Health Sciences Center, Department of Surgery, Texas Tech University, Lubbock, USA

  Kendra P. Rumbaugh

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