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Tandem Repeats in Genes, Proteins, and Disease

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Danny M. Hatters

    1. , Dept. of Biochemistry and Molecular Biol, The University of Melbourne, Parkville, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. Anthony J. Hannan

    1. Florey Neuroscience Institutes, and Mental Health, The University of Melbourne, Parkville, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1017)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins

    • Andrey S. Tsvetkov, D. Michael Ando, Steven Finkbeiner
    Pages 1-20

  3. Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces

    • Kathleen A. Burke, Justin Legleiter
    Pages 21-40

  4. Morphometric Analysis of Huntington’s Disease Neurodegeneration in Drosophila

    • Wan Song, Marianne R. Smith, Adeela Syed, Tamas Lukacsovich, Brett A. Barbaro, Judith Purcell et al.
    Pages 41-57

  5. Size Analysis of Polyglutamine Protein Aggregates Using Fluorescence Detection in an Analytical Ultracentrifuge

    • Saskia Polling, Danny M. Hatters, Yee-Foong Mok
    Pages 59-71

  6. A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins

    • Amy L. Robertson, Stephen P. Bottomley
    Pages 73-83

  7. Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation

    • Yasmin M. Ramdzan, Rebecca Wood, Danny M. Hatters
    Pages 85-93

  8. Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders

    • Emma L. Burrows, Anthony J. Hannan
    Pages 95-104

  9. PCR Amplification and Sequence Analysis of GC-Rich Sequences: Aristaless-Related Homeobox Example

    • May H. Tan, Jozef Gécz, Cheryl Shoubridge
    Pages 105-120

  10. Challenges of “Sticky” Co-immunoprecipitation: Polyalanine Tract Protein–Protein Interactions

    • T. R. Mattiske, May H. Tan, Jozef Gécz, Cheryl Shoubridge
    Pages 121-133

  11. Molecular Pathology of Polyalanine Expansion Disorders: New Perspectives from Mouse Models

    • James N. Hughes, Paul Q. Thomas
    Pages 135-151

  12. Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation

    • Martin L. Duennwald
    Pages 153-161

  13. Immuno-based Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological Samples

    • Gregor P. Lotz, Andreas Weiss
    Pages 163-171

  14. Modeling and Analysis of Repeat RNA Toxicity in Drosophila

    • S. E. Samaraweera, L. V. O’Keefe, C. L. van Eyk, K. T. Lawlor, D. T. Humphreys, C. M. Suter et al.
    Pages 173-192

  15. Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis

    • Mats Holmberg, Ellen A. A. Nollen
    Pages 193-199

  16. Kinetic Analysis of Aggregation Data

    • Regina M. Murphy
    Pages 201-217

  17. A Bioinformatics Method for Identifying Q/N-Rich Prion-Like Domains in Proteins

    • Eric D. Ross, Kyle S. MacLea, Charles Anderson, Asa Ben-Hur
    Pages 219-228

  18. Detecting Soluble PolyQ Oligomers and Investigating Their Impact on Living Cells Using Split-GFP

    • Patrick Lajoie, Erik Lee Snapp
    Pages 229-239

  19. Cell Biological Approaches to Investigate Polyglutamine-Expanded AR Metabolism

    • Lori J. Cooper, Diane E. Merry
    Pages 241-253

  20. Back Matter

    Pages 255-258
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Keywords

About this book

The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences.  Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine.  Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states.  This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular  and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.

Editors and Affiliations

  • , Dept. of Biochemistry and Molecular Biol, The University of Melbourne, Parkville, Australia

    Danny M. Hatters

  • Florey Neuroscience Institutes, and Mental Health, The University of Melbourne, Parkville, Australia

    Anthony J. Hannan

Bibliographic Information

  • Book Title: Tandem Repeats in Genes, Proteins, and Disease

  • Book Subtitle: Methods and Protocols

  • Editors: Danny M. Hatters, Anthony J. Hannan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-438-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-437-1Published: 30 May 2013

  • Softcover ISBN: 978-1-4939-6300-3Published: 23 August 2016

  • eBook ISBN: 978-1-62703-438-8Published: 30 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 258

  • Number of Illustrations: 32 b/w illustrations, 23 illustrations in colour

  • Topics: Human Genetics, Gene Function

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