Methods in Molecular Biology

Structural Genomics

General Applications

Editors: Chen, Yu Wai (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-62703-691-7
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About this book

The field of Structural Genomics has produced many technological advances that transform and accelerate structure solution and analysis. Structural Genomics: General Applications emphasizes the benefits to the wider structural research community. It also reflects the current trend in tackling the more ambitious challenges of studying macromolecular machineries and complexes. Divided into three convenient sections, topics include the cloning and production of proteins for structural studies, experimental methods, and computational methods and data analysis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Structural Genomics: General Applications aims primarily to channel spin-off technologies to the average structural biologist in a small or medium-sized laboratory.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • DisMeta: A Meta Server for Construct Design and Optimization

    Pages 3-16

    Huang, Yuanpeng Janet (et al.)

  • Stable Expression Clones and Auto-Induction for Protein Production in E. coli

    Pages 17-32

    Studier, F. William

  • High-Throughput Expression Screening and Purification of Recombinant Proteins in E. coli

    Pages 33-53

    Saez, Natalie J. (et al.)

  • Medium-Throughput Production of Recombinant Human Proteins: Ligation-Independent Cloning

    Pages 55-72

    Strain-Damerell, Claire (et al.)

  • Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli

    Pages 73-94

    Burgess-Brown, Nicola A. (et al.)

Buy this book

eBook $109.00
price for USA
  • ISBN 978-1-62703-691-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA
  • ISBN 978-1-62703-690-0
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $149.00
price for USA
  • ISBN 978-1-4939-6060-6
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock

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Bibliographic Information

Bibliographic Information
Book Title
Structural Genomics
Book Subtitle
General Applications
Editors
  • Yu Wai Chen
Series Title
Methods in Molecular Biology
Series Volume
1091
Copyright
2014
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-62703-691-7
DOI
10.1007/978-1-62703-691-7
Hardcover ISBN
978-1-62703-690-0
Softcover ISBN
978-1-4939-6060-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 358
Number of Illustrations
17 b/w illustrations, 63 illustrations in colour
Topics