Skip to main content
SpringerLink
Log in

Biomarker Methods in Drug Discovery and Development

  • Book
  • © 2008

Overview

Editors:
  1. Feng Wang

    1. Procter & Gamble Pharmaceutical Inc, Mason, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Broad coverage of proven and practical biomarker techniques currently applied in drug discovery and development
  • Multiple biomarker analysis methods for each of the different molecular levels
  • Real biomarker applications from early discovery to clinical trials
  • Detailed sample handling and preparation techniques to ensure successful biomarker studies

Part of the book series: Methods in Pharmacology and Toxicology (MIPT)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  2. Biomarker Sample Collection and Handling in the Clinical Setting to Support Early-Phase Drug Development

    • Chris B. Russell, Sid Suggs, Kristina M. Robson, Keith Kerkof, Lisa D. Kivman, Kimberly H. Notari et al.
    Pages 1-26

  3. Gene Expression-Based Biomarkers of Drug Safety

    • Eric A.G. Blomme, Scott E. Warder
    Pages 27-49

  4. Profiling Gene Expression Signatures Using Fluorescent Microspheres

    • Suzanne M Torontali, Jorge M Naciff, Kenton D Juhlin, Jay P Tiesman
    Pages 51-62

  5. Real-Time Polymerase Chain Reaction Gene Expression Assays in Biomarker Discovery and Validation

    • Yulei Wang, Catalin Barbacioru, David Keys, Pius Brzoska, Caifu Chen, Kelly Li et al.
    Pages 63-85

  6. SAGE Analysis in Identifying Phenotype Single Nucleotide Polymorphisms

    • Sandro J de Souza, I-Mei Siu, Janete M Cerutti, Gregory J Riggins
    Pages 87-118

  7. Single Nucleotide Polymorphisms and DNA Methylation Analysis Using Pyrosequencing Methods

    • Jinsheng Yu, Sharon Marsh
    Pages 119-140

  8. The Application of Laser Capture Microdissection for the Analysis of Cell-Type-Specific Gene Expression in a Complex Tissue:

    • William C. Okulicz
    Pages 141-169

  9. The Use of Two-Dimensional Gel Electrophoresis for Plasma Biomarker Discovery

    • Brad Jarrold, Alex Varbanov, Feng Wang
    Pages 171-187

  10. Difference In-Gel Electrophoresis: A High-Resolution Protein Biomarker Research Tool

    • David S. Gibson, David Bramwell, Caitriona Scaife
    Pages 189-209

  11. Label-Free Mass Spectrometry-Based Protein Quantification Technologies in Protein Biomarker Discovery

    • Mu Wang, Jin-Sam You, Kerry G. Bemis, Dawn P.G. Fitzpatrick
    Pages 211-230

  12. Top-Down Quantitative Proteomic Analysis Using a Highly Multiplexed Isobaric Mass Tagging Strategy

    • Bing Xie, Wayne F. Patton, Craig E. Parman
    Pages 231-249

  13. SELDI Technology for Identification of Protein Biomarkers

    • Prasad Devarajan, Gary F. Ross
    Pages 251-271

  14. Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens

    • Rachel M. Gonzalez, Susan M. Varnum, Richard C. Zangar
    Pages 273-290

  15. LC-MS Metabonomics Methodology in Biomarker Discovery

    • Xin Lu, Guowang Xu
    Pages 291-315

  16. GC-MS-Based Metabolomics

    • Sally-Ann Fancy, Klaus Rumpel
    Pages 317-340

  17. NMR-Based Metabolomics for Biomarker Discovery

    • Narasimhamurthy Shanaiah, Shucha Zhang, M. Aruni Desilva, Daniel Raftery
    Pages 341-368

  18. Unraveling Glycerophospholipidomes by Lipidomics

    • Kim Ekroos
    Pages 369-384

  19. Back Matter

    Pages 385-396
    Download chapter PDF
Back to top

Keywords

About this book

The pharmaceutical industry has faced many significant challenges since the early 1990s. The fundamental issue that needs to be addressed is how to improve the efficiency of drug discovery and development. The current research and development (R&D) cost for developing a new therapeutic drug is greater than $800 million. Additionally, it takes an average of 12 years to get a new drug to market with an attrition rate greater than 90%. Reviewing the overall pharmaceutical R&D process, it has become clear that many of the drug failures are due to our lack of knowledge in population diversity, which is responsible for differences in drug efficacy and toxicity. In fact, not a single approved drug is 100% safe and efficacious for all patients. For researchers, the key question is: How can we discover biomarkers that can be used to distinguish patients who will respond to the drug without adverse effects from those who will not respond and/or will have adverse effects? There is tremendous urgency to address this question. Biomarkers also fit perfectly with the vision of personalized medicine, the new expectation of medical practice. This is why biomarker research has been a central focus in many research labs across academia, government agencies, and the pharmaceutical industry. There are many different ways to define biomarkers based on molecular properties, applications, and methods.

Reviews

From the reviews:

"... extremely useful to pharmaceutical scientists who are involved in research all the way from early drug discovery through clinical trials." - Doody's Book Review

"The book is … for the bench scientist and is full of helpful advice on how to achieve high quality data using up-to-date technical approaches in genomics, proteomics and metabonomics. … I warmly recommend this … to all practicing toxicologists." (Roy Forster, British Toxicological Society Newsletter, June-August, 2009)

Editors and Affiliations

  • Procter & Gamble Pharmaceutical Inc, Mason, USA

    Feng Wang

Bibliographic Information

  • Book Title: Biomarker Methods in Drug Discovery and Development

  • Editors: Feng Wang

  • Series Title: Methods in Pharmacology and Toxicology

  • DOI: https://doi.org/10.1007/978-1-59745-463-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-934115-23-7Published: 11 March 2008

  • Softcover ISBN: 978-1-61737-925-3Published: 09 November 2010

  • eBook ISBN: 978-1-59745-463-6Published: 13 February 2008

  • Series ISSN: 1557-2153

  • Series E-ISSN: 1940-6053

  • Edition Number: 1

  • Number of Pages: XVIII, 396

  • Number of Illustrations: 79 b/w illustrations, 9 illustrations in colour

  • Topics: Pharmacology/Toxicology, Human Genetics, Cell Biology, Molecular Medicine

Publish with us

Policies and ethics

Back to top

Search

Navigation