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How Free Cationic Polymer Chains Promote Gene Transfection

Authors:

  • Nominated as an outstanding Ph.D. thesis by the Chinese University of Hong Kong
  • Addresses and elucidates a critical but long overlooked question on non-viral gene delivery
  • Greatly deepens our understanding of polymer-mediated gene transfection, representing a paradigm shift
  • Offers new insights into the rational design of next-generation superior polymeric vectors for gene delivery
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Theses (Springer Theses)

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About this book

In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted “proton sponge” model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.

Authors and Affiliations

  • , Department of Chemistry, University of Chicago, Chicago, USA

    Yue Yanan

Bibliographic Information

  • Book Title: How Free Cationic Polymer Chains Promote Gene Transfection

  • Authors: Yue Yanan

  • Series Title: Springer Theses

  • DOI: https://doi.org/10.1007/978-3-319-00336-8

  • Publisher: Springer Cham

  • eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)

  • Copyright Information: Springer International Publishing Switzerland 2013

  • Hardcover ISBN: 978-3-319-00335-1Published: 03 June 2013

  • Softcover ISBN: 978-3-319-34378-5Published: 23 August 2016

  • eBook ISBN: 978-3-319-00336-8Published: 13 May 2013

  • Series ISSN: 2190-5053

  • Series E-ISSN: 2190-5061

  • Edition Number: 1

  • Number of Pages: XVIII, 94

  • Number of Illustrations: 7 b/w illustrations, 38 illustrations in colour

  • Topics: Polymer Sciences, Organic Chemistry, Nucleic Acid Chemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access