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Field-Flow Fractionation in Biopolymer Analysis

Editors: Williams, S. Kim R., Caldwell, Karin D. (Eds.)

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  • Covers the field-flow fractionation (FFF) family of techniques as applied to biopolymer analysis
  • Summarizes FFF technologies and approaches to date
  • Features the work of experts in FFF who contribute their experiences from diverse fields
  • Addresses regulatory approval considerations in FFF methods development
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  • ISBN 978-3-7091-0154-4
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About this book

This is a timely collection of important biomedical applications for a set of separation/characterization techniques that are rapidly gaining popularity due to their wide dynamic range, high resolution, and ability to function in most commonly used solvent systems. Importantly, the field-flow fractionation (FFF) technique has recently emerged as a prominent complement to size exclusion chromatography for protein pharmaceuticals. Fractionation with FFF is gentle and preserves protein structural integrity better than existing alternatives. In the present text, different chapters are written by experts in their respective field of application, who offer comparisons between the FFF techniques and other methods for characterizing their special focus material. Practical guide-lines for successful implementation, such as choice of operating conditions, are offered in conjunction with each application. In addition to new instrumentation and approaches that address important current topics, readers are provided with an overall sense of prior (but timeless) major developments that may be overlooked in literature searches.

 

Reviews

From the reviews:

“I recommend this book to every FFF user and analytical chemist, and to any researcher, post-graduate students, and academics working with biopolymers. … This book … is essential reading for every current and potential FFF user. It discusses, in depth, current and potential applications, the superiority of FFF over other methods … . This book is certainly a precious help for all scientists in the various fields of (biomedical) research, cell biology, food science, membrane technology, and environmental science, to name a few.” (Patrice Castignolles, Analytical and Bioanalytical Chemistry, Vol. 404, 2012)


Table of contents (17 chapters)

Table of contents (17 chapters)
  • Flow FFF – Basics and Key Applications

    Pages 1-21

    Wahlund, Karl-Gustav (et al.)

  • Assessing Protein-Ultrafiltration Membrane Interactions Using Flow Field-Flow Fractionation

    Pages 23-36

    Kassalainen, Galina E. (et al.)

  • Hollow-Fiber Flow Field-Flow Fractionation: A Pipeline to Scale Down Separation and Enhance Detection of Proteins and Cells

    Pages 37-55

    Reschiglian, Pierluigi (et al.)

  • Two-Dimensional Separation for Proteomic Analysis

    Pages 57-71

    Moon, Myeong Hee (et al.)

  • Field-Flow Fractionation in Therapeutic Protein Development

    Pages 73-88

    Pollastrini, Joey (et al.)

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-3-7091-0154-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA
  • ISBN 978-3-7091-0153-7
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA
  • ISBN 978-3-7091-1719-4
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.

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Bibliographic Information

Bibliographic Information
Book Title
Field-Flow Fractionation in Biopolymer Analysis
Editors
  • S. Kim R. Williams
  • Karin D. Caldwell
Copyright
2012
Publisher
Springer-Verlag Wien
Copyright Holder
Springer-Verlag/Wien
eBook ISBN
978-3-7091-0154-4
DOI
10.1007/978-3-7091-0154-4
Hardcover ISBN
978-3-7091-0153-7
Softcover ISBN
978-3-7091-1719-4
Edition Number
1
Number of Pages
X, 306
Topics

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