Metallomics

1. Front Matter

   Pages i-viii

   PDF

2. Overview

   1. Front Matter

      Pages 1-1

      PDF

   2. Metallomics: Integrated Biometal Science

      • Hiroki Haraguchi

      Pages 3-39

3. Analytical Techniques and Strategies Surrounding Metallomics

   1. Front Matter

      Pages 41-41

      PDF

   2. Speciation and Identification of Chalcogen-Containing Metabolites

      • Yasumitsu Ogra

      Pages 43-61

   3. Visualization of Intracellular Elements Using Scanning X-Ray Fluorescence Microscopy

      • Mari Shimura, Lukasz Szyrwiel, Satoshi Matsuyama, Kazuto Yamauchi

      Pages 63-92

   4. Quantitative Elemental Bioimaging Protocol Using Femtosecond-Laser Ablation-ICP-Mass Spectrometry Coupled with Glass Standard Reference Material

      • Yoshiki Makino, Seiya Ohara, Marina Yamada, Sho Mukoyama, Kentaro Hattori, Shuhei Sakata et al.

      Pages 93-106

   5. Single Cell Analysis by Using ICP-MS

      • Shin-ichi Miyashita, Shin-ichiro Fujii, Kaori Shigeta, Kazumi Inagaki

      Pages 107-124

   6. Synchrotron Radiation X-Ray Analysis of Metal-Accumulating Plants

      • Akiko Hokura, Emiko Harada

      Pages 125-145

   7. 77Se NMR Spectroscopy for Speciation Analysis of Selenium Compounds

      • Noriyuki Suzuki, Yasumitsu Ogra

      Pages 147-155

   8. Protein Quantification and Quantitative Phosphorylation Analysis by the Determination of Hetero Atoms (S and P) by Means of nanoHPLC-ICPMS

      • Yoshinari Suzuki, Naoki Furuta

      Pages 157-180

   9. Analysis of Drug Active Pharmaceutical Ingredients and Biomolecules Using Triple Quadrupole ICP-MS

      • Naoki Sugiyama, Yasuyuki Shikamori

      Pages 181-196

   10. Highly Sensitive Analysis of Proteins and Metabolites by Metal Tagging Using LC-ICP-MS

       • Daigo Iwahata

       Pages 197-215

4. Application of Metallomics in Molecular Biology, Medicine and Pharmaceutical Sciences

   1. Front Matter

      Pages 217-217

      PDF

   2. Comprehensive Element Analysis of Prokaryotic and Eukaryotic Cells as well as Organelles by ICP-MS

      • Tomonari Umemura, Yuichiro Matsui, Shinnosuke Sakagawa, Taku Fukai, Eiji Fujimori, Hidetoshi Kumata et al.

      Pages 219-237

   3. Iron Isotope Signature in Red Blood Cell Samples from Japanese Female Donors of Various Ages

      • Yu-ki Tanaka, Kohshiro Takata, Takahiro Kawasaki, Atsuko Shinohara, Kazuko Ishikawa-Takata, Takafumi Hirata

      Pages 239-263

   4. Roles of Zinc Transporters in Cellular Transport of Cadmium and Manganese

      • Seiichiro Himeno, Hitomi Fujishiro

      Pages 265-283

   5. Link Between Metal Homeostasis and Neurodegenerative Diseases: Crosstalk of Metals and Amyloidogenic Proteins at the Synapse

      • Dai Mizuno, Masahiro Kawahara

      Pages 285-314

   6. New Insight into Metallomics in Cognition

      • Atsushi Takeda, Hanuna Tamano

      Pages 315-339

   7. Frontier of Development for Metallodrugs on the Basis of Metallomic Pharmacology and Medicinal Inorganic Chemistry

      • Hiroyuki Yasui, Yutaka Yoshikawa

      Pages 341-364

This book focuses on recent topics in metallomics, a study of the metallome, or metal-containing biomolecules. Metals can induce various physiological and toxicological effects in a very small amounts, in other words, the concentrations of biometals are very low in organisms. Thus, analytical techniques for a trace amount of metal are crucial to understand the biological and toxicological functions of metals.This volume begins with an overview of metallomics including the history and development of the field. Subsequent parts provide basic and advanced techniques for metallomics. Speciation and imaging of metals are basic approaches to reveal the function of the metallome. The applications of speciation using an HPLC hyphenated with inductively coupled plasma mass spectrometry (LC-ICP-MS) and flow cytometry ICP-MS are described. As advanced approaches, the applications using a micro-flow injection-ICP-MS, an ICP-triple quadrupole mass spectrometer, an ICP-sector field mass spectrometer, and an ICP-multi-collector mass spectrometer are mentioned. For the imaging of metals, basic principles and applications of several techniques such as scanning X-ray fluorescence microscopy and ICP-MS equipped with laser ablation (LA-ICP-MS) are presented. Speciation analyses using electrospray ionization mass spectrometry (ESI-MS), X-ray Absorption Spectroscopy (XAS), and nuclear magnetic resonance spectroscopy (NMR) are also introduced. The last part highlights the medical and pharmaceutical applications of metallomics. Molecular biological approaches to reveal the effects of toxic metals, metal functions in brain and neurodegenerative diseases, and metallodrugs are explained. The topic of metal transporters is also presented.

• Metallomics
• Speciation
• Elemental imaging
• Micro-XRF
• NMR
• Stable isotope
• Trace elements
• Metal transporter
• Metallodrugs
• Brain
• Toxicology
• Neurology

“Metallomics: recent analytical techniques and applications seems adequate for Ph.D. students who are trying to approach the topic of metallomics as well as for scientists working in this discipline to update their knowledge of the field. … It provides an overview of fundamental principles, problems and tools for the analysis of biomolecules.” (Maria Montes-Bayón, Analytical and Bioanalytical Chemistry, Vol. 410, 2018)​

• Graduate School of Pharmaceutical Science, Chiba University, Chiba, Japan

  Yasumitsu Ogra

• Graduate School of Science, The University of Tokyo, Tokyo, Japan

  Takafumi Hirata

Yasumitsu Ogra
Professor, Department of Toxicology and Environmental Health, Graduate School of Pharmaceutical Sciences, Chiba University, 1‐8‐1 Inohana, Chuo‐ku, Chiba 260‐8675, Japan


Takafumi Hirata
Professor, Earth and Planetary Sciences, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake‐cho, Sakyo‐ku, Kyoto 606‐8502, Japan

Policies and ethics