The CRISPR/Cas Tool Kit for Genome Editing

1. Front Matter

   Pages i-xii

   PDF

2. An Introduction to Genome Editing Techniques

   • Nayla Munawar, Aftab Ahmad

   Pages 1-28

3. History and Classification of CRISPR/Cas System

   • Zulqurnain Khan, Zulfiqar Ali, Asif Ali Khan, Tahmina Sattar, Ali Zeshan, Tahira Saboor et al.

   Pages 29-52

4. Bioinformatic Tools in CRISPR/Cas Platform

   • Aftab Ahmad, Sidra Ashraf, Humera Naz Majeed, Sabin Aslam, Muhammad Aamir Aslam, Muhammad Salman Mubarik et al.

   Pages 53-111

5. Delivery Methods for CRISPR/Cas Reagents

   • Sidra Ashraf, Nayla Munawar, Muhammad Kashif Zahoor, Amer Jamil, Masooma Hammad, Abdul Ghaffar et al.

   Pages 113-148

6. Reengineering of the CRISPR/Cas System

   • Zulqurnain Khan, Tahmina Sattar, Maria Siddique, Zulfiqar Ali, Asif Ali Khan, Syed Ovais Aftab et al.

   Pages 149-186

7. Beyond Genome Editing: CRISPR Approaches

   • Qaisar Sultan, Sidra Ashraf, Ahmad Munir, Sultan Habibullah Khan, Nayla Munawar, Kamel A. Abd-Elsalam et al.

   Pages 187-218

8. RNA Editing with CRISPR/Cas13

   • Sidra Ashraf, Muhammad Zubair Ghouri, Muhammad Asif Javed, Hassan Zafar, Hazrat Ali, Sameer H. Qari et al.

   Pages 219-254

9. CRISPR for Rewriting Genetic Code

   • Zulqurnain Khan, Asim Razzaq, Ayesha Muneer, Maria Siddique, Umar Iqbal, Muhammad Asif Raza et al.

   Pages 255-283

10. Applications of CRISPR/Cas System in Plants

    • Sultan Habibullah Khan, Hooran Tariq, Iqra Farooq, Hira Tasleeem, Muhammad Zubair Ghouri, Muhammad Salman Mubarik et al.

    Pages 285-309

11. Challenges and Future Prospects of CRISPR Technology

    • Zulqurnain Khan, Sultan Habibullah Khan, Aftab Ahmad

    Pages 311-333

This book discusses CRISPR/Cas- one of the most powerful tools available to scientists for genome editing. CRISPR/Cas is not only a genome editing tool, but researchers have also engineered it for gene regulation, genome imaging, base editing and epigenome regulations. This book describes the entire toolkit for CRISPR/Cas. The opening section gives an introduction to the technique and compares it with other genome editing tools. Further section gives a historical perspective of the tool, along with its detailed classification. The next chapters describe bioinformatic tools in CRISPR/Cas, and delivery methods for CRISPR/Cas. The book also discusses about the applications of CRISPR/Cas beyond genome editing and use of CRISPR for rewriting genetic codes. The book dedicates a section to the use of CRISPR in plants. The book culminates with a chapter on the current status, challenges and shortcomings of the CRISPR/Cas genome editing tool. 


The book would be highly interesting to students and researchers in molecular biology, biochemistry, biotechnology, food science, agriculture and plant sciences.

• CRISPR Based Techniques
• CRISPR Toolkit
• CRISPR/Cas
• Genome Editing
• Genetically Modified Crops

• Department of Biochemistry/US-Pakistan Center for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad, Pakistan

  Aftab Ahmad

• Center of Agricultural Biochemistry and Biotechnology (CABB)/US-Pakistan Center for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad, Pakistan

  Sultan Habibullah Khan

• Institute of Plant Breeding and Biotechnology, Muhammad Nawaz Sharif University of Agriculture, Multan, Pakistan

  Zulqurnain Khan

Dr Aftab Ahmad is currently working as Assistant Professor at Department of Biochemistry/ US-Pakistan Center for Advanced Studies in Agriculture and Food Security (CAS-AFS), University of Agriculture, Faisalabad (UAF), Pakistan. Dr Aftab’s research interests include developing virus resistant and salt tolerant plants using CRISPR/Cas technology. He has published more than 15 research articles in international peer reviewed journals including Journal of Biomedical Sciences, Scientific Reports-Nature, PLOS One, Journal of Translational Medicine, Plant Molecular Biology Reporter, Molecules, Journal of Cereal Science, and Genetic Resources and Crop Evolution. Dr Aftab pursued his PhD and postdocs in Plant Molecular Biology from University of Shizuoka, Japan under Center of Excellence (COE, 21st Century) program. Dr Aftab also served as visiting scientist in department of plant sciences, University of California, Davis, USA. Dr Aftab has been teaching biochemistry and molecular biologyat UAF and his research focuses in the area of CRISPR/Cas based genome editing in plants.


Dr Sultan Habibullah Khan received his PhD from the University of Manchester-England in the field of Plant Sciences in 2004. Since his graduation from UK, Dr Khan has been working as a faculty at the University of Agriculture-Faisalabad (UAF). His postdoctoral experience also includes teaching and research at the University of California-Berkeley and Sultan Qaboos University-Oman. Currently, he is working as the Deputy Chief of Party at the US-Pakistan Center for Advanced Studies in Agriculture and Food Security (CAS-AFS) and a Tenured Associate Professor of Plant Molecular Sciences at the Centre of Agricultural Biochemistry and Biotechnology (CABB), UAF. For the last over 20 years, Dr Khan has been engaged in the fields of plant biotechnology, breeding, genetics and molecular biology. Dr Khan is recipient of national/international scholarships, research productivity award (2014) and over 50 international publications with impact factor.


Dr Zulqurnain Khan is currently working as Assistant Professor in the Institute of Plant Breeding and Biotechnology (IPBB), Muhammad Nawaz Sharif University of Agriculture, Multan (MNSUAM), Pakistan. Dr Khan earned his PhD in Biotechnology from University of Agriculture, Faisalabad (UAF), Pakistan and his research focuses on CRISPR/Cas9 system for development of resistance against begomoviruses using model plants. He has published one book chapter, three review articles, and three research articles in international peer reviewed journals including Journal of Biomedical Sciences, Plant Molecular Biology Reporter and International Journal of Agriculture and Biology. Dr Khan also worked as visiting researcher with group of Professor Caixia Gao at the Institute of Genetics and Developmental Biology (IGDB), Chinese Academy of Sciences (CAS), Beijing, China.

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