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Shotgun Proteomics

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Mónica Carrera

    1. Department of Food Technology, Institute of Marine Research (IIM), Spanish National Research Council (CSIC), Vigo, Spain

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  2. Jesús Mateos

    1. Department of Food Technology, Institute of Marine Research (IIM), Spanish National Research Council (CSIC), Vigo, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2259)

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Table of contents (20 protocols)

  1. Analysis of Postranslational Modifications and Protein Complexes

    1. FTSC-Labeling Coupled with 2DE-LC–MS/MS Analysis of Complex Protein Mixtures for Identification and Relative Quantification of Tissue Carbonylome

      • Lucía Méndez, Lorena Barros, Silvia Muñoz, Isabel Medina
      Pages 227-246

    2. Mass Spectrometry-Based Proteomics for Analysis of Hydrophilic Phosphopeptides

      • Chia-Feng Tsai, Jeffrey S. Smith, Dylan S. Eiger, Kendall Martin, Tao Liu, Richard D. Smith et al.
      Pages 247-257

    3. Rapid Shotgun Phosphoproteomics Analysis

      • Mónica Carrera, Benito Cañas, Daniel Lopez-Ferrer
      Pages 259-268

    4. System-Wide Profiling of Protein Complexes Via Size Exclusion Chromatography– Mass Spectrometry (SEC–MS)

      • Andrea Fossati, Fabian Frommelt, Federico Uliana, Claudia Martelli, Matej Vizovisek, Ludovic Gillet et al.
      Pages 269-294

  2. Shotgun Proteomics Data Processing and Storage

    1. Front Matter

      Pages 295-295
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    2. Qualitative and Quantitative Shotgun Proteomics Data Analysis from Data-Dependent Acquisition Mass Spectrometry

      • Jesse G. Meyer
      Pages 297-308

    3. The jPOST Repository as a Public Data Repository for Shotgun Proteomics

      • Yu Watanabe, Akiyasu C. Yoshizawa, Yasushi Ishihama, Shujiro Okuda
      Pages 309-322

  3. Back Matter

    Pages 323-324
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Keywords

About this book

This edition details a collection of specific shotgun proteomics-based laboratory techniques and applications developed in leading laboratories and proteomics units worldwide. Chapters cover a broad range of topics covering, shotgun proteomics of extracellular vesicles and subcellular structures, shotgun proteomics in non-model organisms, clinical proteomics, food proteomics, analysis of post-translational modifications and protein complexes, and data processing and storage. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Shotgun Proteomics: Methods and Protocols aims to be an up-to-date guide for researchers seeking to understand the proteome of any given biological sample.


Editors and Affiliations

  • Department of Food Technology, Institute of Marine Research (IIM), Spanish National Research Council (CSIC), Vigo, Spain

    Mónica Carrera, Jesús Mateos

Bibliographic Information

  • Book Title: Shotgun Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Mónica Carrera, Jesús Mateos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1178-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1177-7Published: 10 March 2021

  • Softcover ISBN: 978-1-0716-1180-7Published: 10 March 2022

  • eBook ISBN: 978-1-0716-1178-4Published: 09 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 324

  • Number of Illustrations: 10 b/w illustrations, 49 illustrations in colour

  • Topics: Proteomics

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