Overview
- Collects time tested reproducible laboratory protocols including tricks and hints written by experts in the field
- Currently the only book that examines organelle proteomics in depth
Part of the book series: Methods in Molecular Biology (MIMB, volume 432)
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About this book
Proteomic analyses have proven to be an invaluable tool in obtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols provide step-by-step instructions to successfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well as by preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomics allows scientists to gain a vital and important understanding of organelle study.
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Keywords
Table of contents (27 protocols)
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Analysis of Organelles by On-Line Two-Dimensional Liquid Chromatography–Tandem Mass Spectrometry
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Analysis of Organelles, Vesicles, and Protein Machineries
Editors and Affiliations
Bibliographic Information
Book Title: Organelle Proteomics
Editors: Delphine Pflieger, Jean Rossier
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-028-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-779-2Published: 19 February 2008
Softcover ISBN: 978-1-61737-772-3Published: 19 November 2010
eBook ISBN: 978-1-59745-028-7Published: 27 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVII, 421
Topics: Biochemistry, general, Proteomics, Cell Biology